Label-free distinction between p53+/+ and p53-/- colon cancer cells using a graphene based SERS platform

被引：25

作者：

Liang, Owen ^{[1
,3
,4
]}

Wang, Pu ^{[1
]}

Xia, Ming ^{[1
]}

Augello, Catherine ^{[2
]}

Yang, Fan ^{[1
]}

Niu, Gang ^{[3
,4
]}

Liu, Huinan ^{[2
,5
,6
]}

Xie, Ya-Hong ^{[1
,3
,4
,7
]}

机构：

[1] Univ Calif Los Angeles, Dept Mat Sci & Engn, Los Angeles, CA 90095 USA

[2] Univ Calif Riverside, Dept Bioengn, Riverside, CA 92521 USA

[3] Xi An Jiao Tong Univ, Minist Educ, Key Lab, Elect Mat Res Lab, Xian 710049, Shaanxi, Peoples R China

[4] Xi An Jiao Tong Univ, Sch Elect & Informat Engn, Int Ctr Dielect Res, Xian 710049, Shaanxi, Peoples R China

[5] Univ Calif Riverside, Sch Med, Cell Mol & Dev Biol CMDB Program, Riverside, CA 92507 USA

[6] Univ Calif Riverside, Sch Med, Biomed Sci Program, Riverside, CA 92507 USA

[7] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90024 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2018年 / 118卷

关键词：

Graphene; SERS; Colon; Cancer; Label-Free; p53; ENHANCED RAMAN-SCATTERING; SPECTROSCOPY SERS; SURFACE PROTEOME; TISSUES;

D O I：

10.1016/j.bios.2018.07.038

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Surface-Enhanced Raman Scattering (SERS) is used to differentiate two colon cancer cell line HCT 116, that is, to distinguish a TP53 gene knockout cell line (p53 -/-) from a wild type (p53 +/+). A label-free graphene/gold nanopyramid based SERS platform, combined with the multivariate analysis: principal component analysis, is used to profile live, dead, and burst colon cancer cells suspended in simulated body fluid (SBF). The graphene sheet permits SERS hotspot identification and provides a chemical enhancement for the biological constituents. This study found that a unique fingerprint exists for three different states of the cell, burst, live, and dead, which were used to differentiate the p53 +/+ and p53 -/- cell lines. Perceptron with Pocket Algorithm was also coupled with PCA to demonstrate an average of 81% sensitivity and 97% specificity in separating the two cell lines. The demonstration of single gene differentiation shows the great applicable potential of this SERS graphene hybrid platform for cancer diagnosis.

引用

页码：108 / 114

页数：7

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