Single-cell measurements of human neutrophil activation using electrorotation

被引:46
作者
Griffith, AW [1 ]
Cooper, JM [1 ]
机构
[1] Univ Glasgow, Dept Elect & Elect Engn, Bioelect Res Ctr, Glasgow G12 8QQ, Lanark, Scotland
关键词
D O I
10.1021/ac980070c
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have used the technique of cell electrorotation within a microfabricated planar gold electrode array as a means of detecting changes in the physical properties of a single human neutrophil, when activated by the chemotactic factor, phorbol myristate acetate (PIMA). The results, which have been analyzed using a double-shell model to represent the cell and its nucleus, provide an indication of the changes in biophysical parameters that occur during cell activation. The methods used in this study have potential applications in the development of single-cell assays for pharmaceutical screening, as a means of determining rapidly the action of drugs.
引用
收藏
页码:2607 / 2612
页数:6
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