Whole-cell display of Pyrococcus horikoshii glutamate decarboxylase in Escherichia coli for high-titer extracellular gamma-aminobutyric acid production

被引：6

作者：

Somasundaram, Sivachandiran ^{[1
]}

Jeong, Jaehoon ^{[1
]}

Kumaravel, Ashokkumar ^{[1
]}

Hong, Soon Ho ^{[1
]}

机构：

[1] Univ Ulsan, Dept Chem Engn, 93 Daehak Ro, Ulsan 44610, South Korea

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2021年 / 48卷 / 7-8期

基金：

新加坡国家研究基金会;

关键词：

Cell-surface display; Glutamate decarboxylase; Gamma-aminobutyric acid; Outer membrane protein; Thermophilic enzyme; SURFACE; EXPRESSION; PROTEINS; ETHANOL; YEAST;

D O I：

10.1093/jimb/kuab039

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the effect of cell-surface display of glutamate decarboxylase (GadB) on gamma-aminobutyric acid (GABA) production in recombinant Escherichia coli. We integrated GadB from the hyperthermophilic, anaerobic archaeon Pyrococcus horikoshii to the C-terminus of the E. colt outer membrane protein C (OmpC). After 12 hr of culturing GadB-displaying cells, the GABA concentration in the extracellular medium increased to 3.2 el, which is eight times that obtained with cells expressing GadB in the cytosol. To further enhance GABA production, we increased the temperatures of the culture. At 60 degrees C, the obtained GABA concentration was 4.62 g/I after 12 hr of culture, and 5.35 g/l after 24 hr, which corresponds to a yield of 87.7%.

引用

页数：5

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