Immunization with truncated envelope protein of Zika virus induces protective immune response in mice

被引：31

作者：

Han, Jian-Feng ^{[1
]}

Qiu, Yang ^{[1
,3
]}

Yu, Jiu-Yang ^{[2
]}

Wang, Hong-Jiang ^{[1
]}

Deng, Yong-Qiang ^{[1
]}

Li, Xiao-Feng ^{[1
]}

Zhao, Hui ^{[1
]}

Sun, Han-Xiao ^{[2
]}

Qin, Cheng-Feng ^{[1
,3
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, Dept Virol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Jinan Univ, Guangzhou 510632, Guangdong, Peoples R China

[3] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

GENOMIC CHARACTERIZATION; DENGUE VIRUS; INFECTION; ANTIBODY;

D O I：

10.1038/s41598-017-10595-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The global spread of Zika virus (ZIKV) as well as its unexpected link to infant microcephaly have resulted in serious public health concerns. No antiviral drugs against ZIKV is currently available, and vaccine development is of high priority to prepare for potential ZIKV pandemic. In the present study, a truncated E protein with the N-terminal 90% region reserved (E90) from a contemporary ZIKV strain was cloned and expressed in Escherichia coli, purified by a Ni-NTA column, and characterized by Western blotting assays. Immunization with recombinant E90 induced robust ZIKV-specific humoral response in adult BALB/c mice. Passive transfer of the antisera from E90-immunized mice conferred full protection against lethal ZIKV challenge in a neonatal mice model. Our results indicate that recombinant ZIKV E90 described here represents as a promising ZIKV subunit vaccine that deserves further clinical development.

引用

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