Rapid identification of Fusobacterium nucleatum and Fusobacterium necrophorum by fluorescence in situ hybridization

被引：12
|
作者：
Sigge, Anja [1 ]
Essig, Andreas
Wirths, Beate
Fickweiler, Kristina
Kaestner, Nicole
Wellinghausen, Nele
Poppert, Sven
机构：
[1] Univ Hosp Ulm, Dept Med Microbiol & Hyg, D-89081 Ulm, Germany
[2] Univ Leipzig, German Conciliar Lab Anaerob Pathogens, Dept Med Microbiol & Infect Epidemiol, D-04103 Leipzig, Germany
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 58卷 / 02期
关键词：
Fusobacterium; Fusobacterium nucleation; Fusobacterium necrophorum; FISH; 16S rRNA; probe;
D O I：
10.1016/j.diagmicrobio.2007.01.001
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Identification of clinically relevant Fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. A newly developed fluorescence in situ hybridization (FISH) assay allowed reliable and rapid identification of Fusobacterium necrophorum and Fusobacterium nucleatum from culture. Preliminary results show that the method offers the perspective for direct detection of these pathogens in blood cultures and abscess aspirates. (c) 2007 Published by Elsevier Inc.
引用
收藏
页码：255 / 259
页数：5
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