Rapid dissemination and diversity of CTX-M extended-spectrum β-lactamase genes in commensal Escherichia coli isolates from healthy children from low-resource settings in Latin America

被引:128
作者
Pallecchi, Lucia
Bartoloni, Alessandro
Fiorelli, Costanza
Mantella, Antonia
Di Magiio, Tiziana
Garnboa, Herlan
Gotuzzo, Eduardo
Kronvall, Goran
Paradisi, Franco
Rossolini, Gian Maria
机构
[1] Univ Siena, Sez Microbiol, Dipartimento Biol Mol, I-53100 Siena, Italy
[2] Univ Florence, Clin Malattie Infett, Dipartimento Area Crit Med Chirurg, Florence, Italy
[3] Dist Salud Cordillera, Dept Santa Cruz, Camiri, Bolivia
[4] Univ Peruana Cayetano Heredia, Inst Med Trop Alexander Humboldt, Lima, Peru
[5] Karolinska Inst, Dept Microbiol & Tumor Biol MTC, Clin Microbiol, Stockholm, Sweden
关键词
D O I
10.1128/AAC.00026-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A survey carried out in 2005 among members of a healthy population of children living in Bolivia and Peru revealed that fecal carriage of Escherichia coli strains resistant to expanded-spectrum cephalosporins was remarkably increased compared to that observed in the same settings in 2002 (1.7% in 2005 versus 0.1% in 2002). In this work, we demonstrated that this phenomenon was mainly related to the dissemination of CTX-M-type extended-spectrum P-lactamase (ESBL) determinants among commensal E. coli strains. Of 50 ESBL-producing isolates collected in the 2005 survey, 44 harbored a CTX-M-type and 6 an SHV-type (SHV-2 or SHV-12) ESBL. Compared to 2002 results, an increased diversity of CTX-M-type ESBLs was also observed: members of the CTX-M-1 group (CTX-M-15) emerged in Bolivia (where only CTX-M-2 was observed in 2002), while members of the CTX-M-9 group (CTX-M-14 and CTX-M-24) emerged in Peru (where only CTX-M-15 and CTX-M-2 were observed in 2002). A new CTX-M-2 variant named CTX-M-56 was also detected. Molecular characterization of the CTX-M-producing isolates and gene transfer experiments suggested that different mechanisms could be involved in the spreading of different CTX-M group determinants and revealed that additional resistance determinants for non-p-lactam antibiotics were preferentially carried by plasmids encoding certain CTX-M variants (CTX-M-15 and variants of the CTX-M-2 group). Three CTX-M-15-encoding conjugative plasmids from Peruvian isolates carried the new fluoroquinolone resistance gene aac(6')-Ib-cr. To our best knowledge, this is the first report of the detection of aac(6')-Ib-cr in Latin America.
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页码:2720 / 2725
页数:6
相关论文
共 30 条
[11]   Identification of plasmids by PCR-based replicon typing [J].
Carattoli, A ;
Bertini, A ;
Villa, L ;
Falbo, V ;
Hopkins, KL ;
Threlfall, EJ .
JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 63 (03) :219-228
[12]   Rapid and simple determination of the Escherichia coli phylogenetic group [J].
Clermont, O ;
Bonacorsi, S ;
Bingen, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4555-4558
[13]  
Clinical and Laboratory Standards Institute, 2006, PERF STAND ANT DISK
[14]   DNA sequence analysis of the genetic environment of various blaCTX-M genes [J].
Eckert, C ;
Gautier, V ;
Arlet, G .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 57 (01) :14-23
[15]   Replicon typing of Plasmids carrying CTX-M or CMY β-lactamases circulating among Salmonella and Escherichia coli isolates [J].
Hopkins, Katie L. ;
Liebana, Ernesto ;
Villa, Laura ;
Batchelor, Miranda ;
Threlfall, E. John ;
Carattoli, Alessandra .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (09) :3203-3206
[16]   Mechanisms of disease:: The new β-lactamases [J].
Jacoby, GA ;
Munoz-Price, LS .
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (04) :380-391
[17]   Trends in production of extended-spectrum β-lactamases among enterobacteria of medical interest:: Report of the second Italian nationwide survey [J].
Luzzaro, Francesco ;
Mezzatesta, Marilina ;
Mugnaioli, Claudia ;
Perilli, Mariagrazia ;
Stefani, a Stefani ;
Amicosante, Gianfranco ;
Rossolini, Gian Maria ;
Toniolo, Antonio .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (05) :1659-1664
[18]   Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans [J].
Pacheco, ABF ;
Guth, BEC ;
Soares, KCC ;
Nishimura, L ;
DeAlmeida, DF ;
Ferreira, LCS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) :1521-1525
[19]   Identification of CTX-M-14 extended-spectrum β-lactamase in clinical isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea [J].
Pai, H ;
Choi, EH ;
Lee, HJ ;
Hong, JY ;
Jacoby, GA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) :3747-3749
[20]   Detection of CTX-M-type β-lactamase genes in fecal Escherichia coli isolates from healthy children in Bolivia and Peru [J].
Pallecchi, L ;
Malossi, M ;
Mantella, A ;
Gotuzzo, E ;
Trigoso, C ;
Bartoloni, A ;
Paradisi, F ;
Kronvall, G ;
Rossolini, GM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (12) :4556-4561