Nitrite infusion increases cerebral blood flow and decreases mean arterial blood pressure in rats: A role for red cell NO

被引:42
作者
Ritkind, Joseph M.
Nagababu, Enika
Barbiro-Michaely, Efrat
Ramasamy, Somasundaram
Pluta, Ryszard M.
Mayevsky, Avraham
机构
[1] NIA, Mol Dynam Sect, Baltimore, MD 21224 USA
[2] NINDS, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA
[3] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel
来源
NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2007年 / 16卷 / 04期
基金
以色列科学基金会; 美国国家卫生研究院;
关键词
cerebral circulation; erythrocytm nitric oxide; nitrite; vasodilation;
D O I
10.1016/j.niox.2007.04.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been proposed that the reduction of nitrite by red cells producing NO plays a role in the regulation of vascular tone. This hypothesis was investigated in rats by measuring the effect of nitrite infusion on mean arterial blood pressure (MAP), cerebral blood flow (CBF) and cerebrovascular resistance (CVR) in conjunction with the accumulation of red cell NO. The relative magnitude of the effects on MAP and CBF as well as the time dependent changes during nitrite infusion are used to distinguish between the effects on the peripheral circulation and the effects on the cerebral circulation undergoing cerebral autoregulation. The nitrite infusion was found to reverse the 96% increase in MAP and the 13% decrease in CBF produced by L-NAME inhibition of e-NOS. At the same time there was a 20-fold increase in oxygen stable red cell NO. Correlations of the red cell NO for individual rats support a role for red cell nitrite reduction in regulating vascular tone in both the peripheral and the cerebral circulation. Furthermore, data obtained prior to treatment is consistent with a contribution of red cell reduced nitrite in regulating vascular tone even under normal conditions. (c) 2007 Published by Elsevier Inc.
引用
收藏
页码:448 / 456
页数:9
相关论文
共 41 条
[31]  
Reutov VP, 1998, BIOCHEMISTRY-MOSCOW+, V63, P874
[32]   Nitric oxide redox reactions and red cell biology [J].
Rifkind, Joseph M. ;
Nagababu, Enika ;
Ramasamy, Somasundaram .
ANTIOXIDANTS & REDOX SIGNALING, 2006, 8 (7-8) :1193-1203
[33]   Nitrite reductase activity of sol-gel-encapsulated deoxyhemoglobin - Influence of quaternary and tertiary structure [J].
Roche, Camille J. ;
Dantsker, David ;
Samuni, Uri ;
Friedman, Joel M. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (48) :36874-36882
[34]   Evaluation of the ratio of cerebral blood flow to cerebral blood volume as an index of local cerebral perfusion pressure [J].
Schumann, P ;
Touzani, O ;
Young, AR ;
Baron, JC ;
Morello, R ;
MacKenzie, ET .
BRAIN, 1998, 121 :1369-1379
[35]   Cerebral pressure-flow relations in hypertensive elderly humans: transfer gain in different frequency domains [J].
Serrador, JM ;
Sorond, FA ;
Vyas, M ;
Gagnon, M ;
Iloputaife, ID ;
Lipsitz, LA .
JOURNAL OF APPLIED PHYSIOLOGY, 2005, 98 (01) :151-159
[36]   Nitric oxide mediation of chemoregulation but not autoregulation of cerebral blood flow in primates [J].
Thompson, BG ;
Pluta, RM ;
Girton, ME ;
Oldfield, EH .
JOURNAL OF NEUROSURGERY, 1996, 84 (01) :71-78
[37]  
WADHWANI KC, 1996, LASER DOPPLER BLOOD, P265
[38]   SPINAL-CORD BLOOD-FLOW MEASURED WITH MICROSPHERES FOLLOWING SPINAL-CORD INJURY IN THE RAT [J].
WALLACE, MC ;
TATOR, CH .
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES, 1986, 13 (02) :91-96
[39]  
WERNER C, 1993, ANESTH ANALG, V76, P971
[40]   Kinetics and mechanism of the reversible binding of nitric oxide to reduced cobalamin B12r (Cob(II)alamin) [J].
Wolak, M ;
Zahl, A ;
Schneppensieper, T ;
Stochel, G ;
van Eldik, R .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (40) :9780-9791