MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor

被引：93
作者：
Zhu, Li-Hua [1 ,2 ,3 ]
Liu, Tao [1 ,2 ]
Tang, Hua [1 ,2 ]
Tian, Rui-Qing [1 ,2 ]
Su, Chang [1 ,2 ]
Liu, Min [1 ,2 ]
Li, Xin [1 ,2 ]
机构：
[1] Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin 300070, Peoples R China
[2] Tianjin Med Univ, Basic Med Sch, Tianjin, Peoples R China
[3] N China Coal Med Coll, Dept Pathobiol, Biosci Fac, Iangshan, Peoples R China
来源：
FEBS JOURNAL | 2010年 / 277卷 / 18期
基金：
中国国家自然科学基金;
关键词：
cell growth; gastric adenocarcinoma; IL6R; miR-23a; target gene; HEPATOCELLULAR-CARCINOMA; EXPRESSION PATTERNS; IDENTIFICATION; DEREGULATION; MICROARRAY; CLUSTER; GENES;
D O I：
10.1111/j.1742-4658.2010.07773.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs are an evolutionarily conserved class of endogenous noncoding RNAs that modulate gene expression at the post-transcriptional level. Recently, microRNA-23a (miR-23a) has been found to function as a growth-promoting and antiapoptotic factor in hepatocellular carcinoma cells. Our previous study showed that miR-23a was significantly upregulated in gastric adenocarcinoma tissues. In this study, we found that miR-23a promoted the proliferative potential of gastric adenocarcinoma cell line MGC803. We also identified IL6R as a direct target gene for miR-23a using a fluorescent reporter assay. The mRNA and protein levels of IL6R were both inversely correlated with the miR-23a expression level. Our results demonstrate that miR-23a can target IL6R and promote the growth activity of gastric adenocarcinoma cells in vitro. The downregulation of IL6R by miR-23a may explain why the suppression of miR-23a can inhibit gastric cancer cell proliferation.
引用
收藏
页码：3726 / 3734
页数：9
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