Genetic variability of soybean bradyrhizobia populations under different soil managements

被引：21

作者：

Bizarro, Mariel Josue ^{[2
]}

Giongo, Adriana ^{[1
]}

Vargas, Luciano Kayser ^{[3
]}

Wurdig Roesch, Luiz Fernando ^{[4
]}

Gano, Kelsey Annette ^{[1
]}

Saccol de Sa, Enilson Luiz ^{[2
]}

Pereira Passaglia, Luciane Maria ^{[5
]}

Selbach, Pedro Alberto ^{[2
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Univ Fed Rio Grande do Sul, Fac Agron, Dept Ciencia Solo, BR-91501970 Porto Alegre, RS, Brazil

[3] Fundacao Estadual Pesquisa Agropecuaria, BR-90130060 Porto Alegre, RS, Brazil

[4] Univ Fed Pampa, BR-97300000 Sao Gabriel, RS, Brazil

[5] Univ Fed Rio Grande do Sul, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil

来源：

BIOLOGY AND FERTILITY OF SOILS | 2011年 / 47卷 / 03期

关键词：

Bradyrhizobium; Soil managements; Serological characterization; rep-PCR; POLYMERASE CHAIN-REACTION; BEAN PHASEOLUS-VULGARIS; SOUTHERN BRAZIL; DIVERSITY; RHIZOBIA; STRAINS; L; JAPONICUM; SEQUENCES; BACTERIA;

D O I：

10.1007/s00374-010-0512-6

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Due to their ecologic and economic importance, bradyrhizobia have been extensively studied in recent years. Since 1992, Bradyrhizobium elkanii SEMIA 587 and SEMIA 5019 and Bradyrhizobium japonicum SEMIA 5079 and SEMIA 5080 have been widely used in most Brazilian soybean fields. The objective of this work was to estimate the genetic variability of bradyrhizobial isolates recovered from soils under rhizobial inoculation and different soil managements. Only 25% of the isolates demonstrated high similarities to the original strains, and a strong correlation was obtained between the bradyrhizobial genetic variability and soil management. A high level of genetic diversity was observed both within isolates (H = 5.46) as well as among the different soil practices. Soil under no-tillage presented a higher bradyrhizobia diversity compared with bradyrhizobia isolated from soil under conventional tillage. Serological characterization also indicated that B. elkanii strains SEMIA 587 and SEMIA 5019 were more competitive and presented a higher nodular occupancy capacity than strains belonging to B. japonicum species in Southern Brazilian soils.

引用

页码：357 / 362

页数：6

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