Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

被引：75

作者：

Fang, Tao ^{[1
]}

Lu, Xiaotang ^{[2
,3
]}

Berger, Daniel ^{[2
,3
]}

Gmeiner, Christina ^{[2
,3
]}

Cho, Julia ^{[4
]}

Schalek, Richard ^{[2
,3
]}

Ploegh, Hidde ^{[1
]}

Lichtman, Jeff ^{[2
,3
]}

机构：

[1] Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA

[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[3] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA

[4] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

来源：

NATURE METHODS | 2018年 / 15卷 / 12期

关键词：

VHH;

D O I：

10.1038/s41592-018-0177-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

引用

页码：1029 / +

页数：8

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