Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

被引：75

作者：

Fang, Tao ^{[1
]}

Lu, Xiaotang ^{[2
,3
]}

Berger, Daniel ^{[2
,3
]}

Gmeiner, Christina ^{[2
,3
]}

Cho, Julia ^{[4
]}

Schalek, Richard ^{[2
,3
]}

Ploegh, Hidde ^{[1
]}

Lichtman, Jeff ^{[2
,3
]}

机构：

[1] Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA

[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[3] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA

[4] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

来源：

NATURE METHODS | 2018年 / 15卷 / 12期

关键词：

VHH;

D O I：

10.1038/s41592-018-0177-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

引用

页码：1029 / +

页数：8

共 22 条

[1]

Antos J. M, 2017, CURR PROTOC PROTEIN, V89

[2] The activity of myeloid cell-specific VHH immunotoxins is target-, epitope-, subset- and organ dependent [J].

Bachran, Christopher ;

Schroeder, Matthias ;

Conrad, Lena ;

Cragnolini, Juan J. ;

Tafesse, Fikadu G. ;

Helming, Laura ;

Ploegh, Hidde L. ;

Swee, Lee Kim .

SCIENTIFIC REPORTS, 2017, 7

[3] THE METABOTROPIC GLUTAMATE-RECEPTOR (MGLUR1-ALPHA) IS CONCENTRATED AT PERISYNAPTIC MEMBRANE OF NEURONAL SUBPOPULATIONS AS DETECTED BY IMMUNOGOLD REACTION [J].

BAUDE, A ;

NUSSER, Z ;

ROBERTS, JDB ;

MULVIHILL, E ;

MCILHINNEY, RAJ ;

SOMOGYI, P .

NEURON, 1993, 11 (04) :771-787

[4]

Chung K, 2013, NAT METHODS, V10, P508, DOI [10.1038/NMETH.2481, 10.1038/nmeth.2481]

[5] Mapping Synapses by Conjugate Light-Electron Array Tomography [J].

Collman, Forrest ;

Buchanan, Joann ;

Phend, Kristen D. ;

Micheva, Kristina D. ;

Weinberg, Richard J. ;

Smith, Stephen J. .

JOURNAL OF NEUROSCIENCE, 2015, 35 (14) :5792-5807

[6] Generation of Immunity against Pathogens via Single-Domain Antibody-Antigen Constructs [J].

Duarte, Joao N. ;

Cragnolini, Juan J. ;

Swee, Lee Kim ;

Bilate, Angelina M. ;

Bader, Justin ;

Ingram, Jessica R. ;

Rashidfarrokhi, Ali ;

Fang, Tao ;

Schiepers, Arien ;

Hanke, Leo ;

Ploegh, Hidde L. .

JOURNAL OF IMMUNOLOGY, 2016, 197 (12) :4838-4847

[7]

Fang T., 2018, PROTOC EXCH, DOI [10.1038/protex.2018.108, DOI 10.1038/PROTEX.2018.108]

[8] Targeted antigen delivery by an anti-class II MHC VHH elicits focused αMUC1(Tn) immunity [J].

Fang, Tao ;

Van Elssen, Catharina H. M. J. ;

Duarte, Joao N. ;

Guzman, Jonathan S. ;

Chahal, Jasdave S. ;

Ling, Jingjing ;

Ploegh, Hidde L. .

CHEMICAL SCIENCE, 2017, 8 (08) :5591-5597

[9] Structurally Defined aMHC-II Nanobody-Drug Conjugates: A Therapeutic and Imaging System for B-Cell Lymphoma [J].

Fang, Tao ;

Duarte, Joao N. ;

Ling, Jingjing ;

Li, Zeyang ;

Guzman, Jonathan S. ;

Ploegh, Hidde L. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (07) :2416-2420

[10] Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP [J].

Feng, GP ;

Mellor, RH ;

Bernstein, M ;

Keller-Peck, C ;

Nguyen, QT ;

Wallace, M ;

Nerbonne, JM ;

Lichtman, JW ;

Sanes, JR .

NEURON, 2000, 28 (01) :41-51

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