Acid-base response of bacterial suspensions

被引：2

作者：

Rius, N ^{[1
]}

Sole, M ^{[1
]}

Loren, JG ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Dept Microbiol & Parasitol Sanitaries, Div Ciencies Salut, E-08028 Barcelona, Spain

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 21卷 / 1-2期

关键词：

acid-base response; bacterial suspensions; chemoheterotrophic bacteria;

D O I：

10.1038/sj.jim.2900562

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The response of suspensions of non-proliferating bacteria to external pH ranging from 3.6 to 9.9 was examined. The acid-base response is affected by the number of viable cells in the suspensions and culture conditions (aerobiosis or anaerobiosis) but not by culture time, composition of the culture medium or the age of suspensions. The results with carbonylcyanide m-chlorophenylhydrazone-and N, N'- dicyclohexylcarbodiimide-treated cells indicated that passive proton conductance and proton translocation by ATP could be involved in this response, pH profiles of suspensions of 18 chemoheterotrophic Gram-negative and Gram-positive bacteria support the hypothesis that the proton gradient generated by an alkali or an acid shift could be dissipated by bacteria with respiratory metabolism.

引用

页码：65 / 74

页数：10

共 32 条

[1] UNCOUPLING BY ACETIC-ACID LIMITS GROWTH OF AND ACETOGENESIS BY CLOSTRIDIUM-THERMOACETICUM [J].