Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy

被引：29
|
作者：
Spitz, J. -A. [1 ,3 ]
Yasukuni, R. [3 ]
Sandeau, N. [2 ]
Takano, M. [3 ]
Vachon, J. -J. [3 ]
Meallet-Renault, R. [3 ]
Pansu, R. B. [3 ]
机构：
[1] Univ Paris Sud, CNRS, ENS Cacham, Inst Alembert,LBPA, F-94230 Cachan, France
[2] Univ Paris Sud, CNRS, ENS Cacham, Inst Alembert,LPQM, F-94230 Cachan, France
[3] Univ Paris Sud, CNRS, ENS Cacham, Inst Alembert,PPSM, F-94230 Cachan, France
来源：
JOURNAL OF MICROSCOPY-OXFORD | 2008年 / 229卷 / 01期
关键词：
anisotropy; FLIM; fluorescence; quadrant anode; single-photon counting; tr-FAIM; wide-field imaging;
D O I：
10.1111/j.1365-2818.2007.01873.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
A scanning-less single-photon counting system for FLIM and fluorescence anisotropy wide-field imaging is described and characterized in this paper. The two polarizations of the fluorescence are divided by a Glan prism and acquired at the same time by the Q(A) detector. Fluorescence decay profiles can be reconstructed for any desired area up to each pixel and used to calculate time-resolved fluorescence anisotropy decays.
引用
收藏
页码：104 / 114
页数：11
相关论文
共 49 条
  • [1] Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting
    Hirvonen, Liisa M.
    Nedbal, Jakub
    Almutairi, Norah
    Phillips, Thomas A.
    Becker, Wolfgang
    Conneely, Thomas
    Milnes, James
    Cox, Susan
    Sturzenbaum, Stephen
    Suhling, Klaus
    JOURNAL OF BIOPHOTONICS, 2020, 13 (02)
  • [2] Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy
    Suhling, Klaus
    Hirvonen, Liisa M.
    Becker, Wolfgang
    Smietana, Stefan
    Netz, Holger
    Milnes, James
    Conneely, Thomas
    Le Marois, Alix
    Jagutzki, Ottmar
    Festy, Fred
    Petrasek, Zdenek
    Beeby, Andrew
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2019, 942
  • [3] Wide-field photon counting imaging for fluorescence microscopy
    Sergent, Nicolas
    Levitt, James A.
    Green, Mark A.
    Suhling, Klaus
    ADVANCED PHOTON COUNTING TECHNIQUES II, 2007, 6771
  • [4] Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging
    Intes, Xavier
    WAVELETS AND SPARSITY XVII, 2017, 10394
  • [5] Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging
    Pian, Qi
    Yao, Ruoyang
    Sinsuebphon, Nattawut
    Intes, Xavier
    NATURE PHOTONICS, 2017, 11 (07) : 411 - +
  • [6] Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution
    Hirvonen, Liisa M.
    Festy, Frederic
    Suhling, Klaus
    OPTICS LETTERS, 2014, 39 (19) : 5602 - 5605
  • [7] Wide-field photon counting fluorescence lifetime imaging microscopy: application to photosynthesizing systems
    Zdeněk Petrášek
    Hann-Jörg Eckert
    Klaus Kemnitz
    Photosynthesis Research, 2009, 102 : 157 - 168
  • [8] Wide-field photon counting fluorescence lifetime imaging microscopy: application to photosynthesizing systems
    Petrasek, Zdenek
    Eckert, Hann-Joerg
    Kemnitz, Klaus
    PHOTOSYNTHESIS RESEARCH, 2009, 102 (2-3) : 157 - 168
  • [9] A space- and time-resolved single-photon counting detector for fluorescence microscopy and spectroscopy
    Michalet, X.
    Siegmund, O. H. W.
    Vallerga, J. V.
    Jelinsky, P.
    Millaud, J. E.
    Weiss, S.
    ULTRASENSITIVE AND SINGLE-MOLECULE DETECTION TECHNOLOGIES, 2006, 6092
  • [10] Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector
    Michalet, X.
    Siegmund, O. H. W.
    Vallerga, J. V.
    Jelinsky, P.
    Pinaud, F. F.
    Millaud, J. E.
    Weiss, S.
    ADVANCED PHOTON COUNTING TECHNIQUES, 2006, 6372
12345