The Effect of Low Oxygen During the Early Phases of Sperm Freezing in Stallions With Low Progressive Motility: Can We Improve Post-Thaw Motility of Stallion Sperm?

被引：11
作者：
Darr, Christa [1 ]
Martorana, Kelly [1 ]
Scanlan, Tawny [1 ]
Meyers, Stuart [1 ]
机构：
[1] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, 1089 Vet Med Dr,Bldg VM3B, Davis, CA 95616 USA
来源：
JOURNAL OF EQUINE VETERINARY SCIENCE | 2016年 / 42卷
关键词：
Stallion; Cryopreservation; Oxidative stress; Sperm; Oxyrase; MOUSE SPERM; OXYRASE; SPERMATOZOA; GLYCEROL; SURVIVAL; DILUTION;
D O I：
10.1016/j.jevs.2016.03.022
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The objective of this study was to determine the effectiveness of oxygen removal in minimizing oxidative stress to improve fertile longevity of equine spermatozoa. Stallion ejaculates (n = 6) were treated with increasing doses of the oxygen scavenger Oxyrase (0.6 U/mL, 1.2 U/mL, 2.4 U/mL, and 5.0 U/mL). Samples were cryopreserved, thawed, and analyzed for motility over 24 hours at ambient temperature. These data suggest a role of excessive oxygen in the reduced motility of cryopreserved samples as 2.4 U/mL Oxyrase minimized the loss of post-thaw motility over the other doses and control treatments. Oxygen, a key component of reactive oxygen species generation, is directly involved in oxidative damage, and its removal may trigger metabolic changes that are more suited for post-thaw motility maintenance. Removal of oxygen from semen before cryopreservation may promote increased post-thaw fertility and may have the potential to overcome stallion variability in cryosurvival. (C) 2016 Elsevier Inc. All rights reserved.
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页码：44 / 51
页数：8
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