Granzyme-B-containing lymphocyte involvement in epidermal injury in graft-versus-host disease

被引：17
作者：
Higaki, Y
Yamada, O
Okamura, T
Mizoguchi, H
Kawashima, M
机构：
[1] Tokyo Womens Med Univ, Dept Dermatol, Shinjuku Ku, Tokyo 1628666, Japan
[2] Tokyo Womens Med Univ, Dept Haematol, Tokyo 1628666, Japan
来源：
DERMATOLOGY | 2001年 / 202卷 / 02期
关键词：
graft-versus-host disease; perforin; granzyme B; apoptosis; cytotoxic T lymphocytes;
D O I：
10.1159/000051606
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
Background: Skin lesions from graft-versus-host disease (GVHD) show histological features of epidermal cell death with lymphocyte infiltration, Perforin and granzyme B are involved in the process of apoptosis induced by cytotoxic T lymphocytes (CTL). Objective: To elucidate the role of CTL in the mechanism of epidermal injury in GVHD, Methods: We studied immunohistochemical staining far granzyme B and perforin in the skin lesions of 8 patients who developed GVHD after bone marrow transplantation. Results: Granzyme-B-positive lymphocytes were CD8 positive and were observed in the epidermis of 3 out of 6 specimens in acute GVHD, and of 5 specimens of chronic GVHD except for 1 sclerotic type in which it was negative, Perforin-positive lymphocytes were observed in the epidermis of the specimens from 1 acute and 1 chronic GVHD, Conclusions: Granzyme B derived from CTL may be involved in the mechanisms of epidermal injury in GVHD. Copyright (C) 2001 S.Kaiger AG, Basel.
引用
收藏
页码：94 / 98
页数：5
相关论文
共 18 条
[1]   PERFORIN AND GRANZYME-B EXPRESSION IS ASSOCIATED WITH SEVERE ACUTE REJECTION - EVIDENCE FOR IN-SITU LOCALIZATION IN ALVEOLAR LYMPHOCYTES OF LUNG-TRANSPLANTED PATIENTS [J].
CLEMENT, MV ;
LEGROSMAIDA, S ;
ISRAELBIET, D ;
CARNOT, F ;
SOULIE, A ;
REYNAUD, P ;
GUILLET, J ;
GANDJBAKCH, I ;
SASPORTES, M .
TRANSPLANTATION, 1994, 57 (03) :322-326
[2]   GRAFT VERSUS HOST REACTION - ULTRASTRUCTURAL STUDY [J].
DEDOBBELEER, GD ;
LEDOUXCORBUSIER, MH ;
ACHTEN, GA .
ARCHIVES OF DERMATOLOGY, 1975, 111 (12) :1597-1602
[3]   Involvement of TNF-alpha secreting macrophages in lethal forms of human graft-versus-host disease [J].
Facon, T ;
Jouet, JP ;
NoelWalter, MP ;
Bloget, F ;
Bauters, F ;
Janin, A .
BONE MARROW TRANSPLANTATION, 1997, 20 (06) :511-515
[4]   The role of granzyme B in murine models of acute graft-versus-host disease and graft rejection [J].
Graubert, TA ;
Russell, JH ;
Ley, TJ .
BLOOD, 1996, 87 (04) :1232-1237
[5]   Perforin/granzyme-dependent and independent mechanisms are both important for the development of graft-versus-host disease after murine bone marrow transplantation [J].
Graubert, TA ;
DiPersio, JF ;
Russell, JH ;
Ley, TJ .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (04) :904-911
[6]   Differential effects of anti-Fas ligand and anti-tumor necrosis factor α antibodies on acute graft-versus-host disease pathologies [J].
Hattori, K ;
Hirano, T ;
Miyajima, H ;
Yamakawa, N ;
Tateno, M ;
Oshimi, K ;
Kayagaki, N ;
Yagita, H ;
Okumura, K .
BLOOD, 1998, 91 (11) :4051-4055
[7]  
JANINMERCIER A, 1981, ACTA DERM-VENEREOL, V61, P187
[8]   Molecular mechanisms of lymphocyte-mediated cytotoxicity and their role in immunological protection and pathogenesis in vivo [J].
Kagi, D ;
Ledermann, B ;
Burki, K ;
Zinkernagel, RM ;
Hengartner, H .
ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :207-232
[9]   Apoptosis is the mode of keratinocyte death in cutaneous graft-versus-host disease [J].
Langley, RGB ;
Walsh, N ;
Nevill, T ;
Thomas, L ;
Rowden, G .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1996, 35 (02) :187-190
[10]   GRANZYME-B AND PERFORIN CAN BE USED AS PREDICTIVE MARKERS OF ACUTE REJECTION IN HEART-TRANSPLANTATION [J].
LEGROSMAIDA, S ;
SOULIE, A ;
BENVENUTI, C ;
WARGNIER, A ;
VALLEE, N ;
BERTHOU, C ;
GUILLET, J ;
SASPORTES, M ;
SIGAUX, N .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (01) :229-233
12