Study of the Behavior of Lysozyme Oligomers in Solutions by the Molecular Dynamics Method

被引:14
作者
Kordonskaya, Yu. V. [1 ]
Timofeev, V. I. [1 ,2 ]
Dyakova, Yu. A. [1 ,2 ]
Marchenkova, M. A. [1 ,2 ]
Pisarevsky, Yu. V. [1 ,2 ]
Podshivalov, D. D. [1 ,2 ,3 ]
Kovalchuk, M. V. [1 ,2 ,4 ]
机构
[1] Russian Acad Sci, Shubnikov Inst Crystallog, Fed Sci Res Ctr Crystallog & Photon, Moscow 119333, Russia
[2] Natl Res Ctr, Kurchatov Inst, Moscow 123182, Russia
[3] Moscow MV Lomonosov State Univ, Moscow 119991, Russia
[4] St Petersburg State Univ, St Petersburg 199034, Russia
基金
俄罗斯基础研究基金会;
关键词
INITIAL CRYSTALLIZATION STAGE; X-RAY-SCATTERING; PROTEIN;
D O I
10.1134/S1063774518060196
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
The dynamics of octamers of two types, which form (via translation) a tetragonal lysozyme crystal lattice, has been simulated on a 100-ns path with and without a precipitant. It is shown that one of the octamers under study is stable in the presence of a precipitant, whereas the other octamer dissociates into components both in the presence and in the absence of a precipitant. The results obtained not only confirmed the results of previous measurements of lysozyme solutions with NaCl precipitant by small-angle X-ray neutron scattering but also made it possible to establish the type of the octamer forming in the crystallization solution.
引用
收藏
页码:947 / 950
页数:4
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