Overexpression of Mfsd2a attenuates blood brain barrier dysfunction via Cav-1/Keap-1/Nrf-2/HO-1 pathway in a rat model of surgical brain injury

被引：37

作者：

Ocak, Pinar Eser ^{[1
,2
]}

Ocak, Umut ^{[1
,3
,4
]}

Sherchan, Prativa ^{[1
]}

Gamdzyk, Marcin ^{[1
]}

Tang, Jiping ^{[1
]}

Zhang, John H. ^{[1
,5
,6
,7
]}

机构：

[1] Loma Linda Univ, Sch Med, Dept Physiol & Pharmacol, Loma Linda, CA 92354 USA

[2] Uludag Univ, Sch Med, Dept Neurosurg, TR-16120 Bursa, Turkey

[3] Univ Hlth Sci, Bursa Yuksek Ihtisas Training & Res Hosp, Dept Emergency Med, TR-16310 Bursa, Turkey

[4] Bursa City Hosp, Dept Emergency Med, TR-16110 Bursa, Turkey

[5] Loma Linda Univ, Sch Med, Dept Anesthesiol, Loma Linda, CA 92354 USA

[6] Loma Linda Univ, Sch Med, Dept Neurol, Loma Linda, CA 92354 USA

[7] Loma Linda Univ, Sch Med, Dept Neurosurg, Loma Linda, CA 92354 USA

来源：

EXPERIMENTAL NEUROLOGY | 2020年 / 326卷

基金：

美国国家卫生研究院;

关键词：

Blood brain barrier; Brain edema; Cav-1; Caveolae; Mfsd2a; Surgical brain injury; EXPRESSION; CAVEOLAE; DAMAGE; PATHOPHYSIOLOGY; COMPLICATIONS; PERMEABILITY; INHIBITION; CLAUDIN-5; PROTECTS; OCCLUDIN;

D O I：

10.1016/j.expneurol.2020.113203

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Introduction: Disruption of the blood brain barrier (BBB) and subsequent cerebral edema formation is one of the major adverse effects of brain surgery, leading to postoperative neurological dysfunction. Recently, Mfsd2a has been shown to have a crucial role for the maintenance of BBB functions. In this study, we aimed to evaluate the role of Mfsd2a on BBB disruption following surgical brain injury (SBI) in rats. Materials and methods: Rats were subjected to SBI by partial resection of the right frontal lobe. To evaluate the effect of Mfsd2a on BBB permeability and neurobehavior outcome following SBI, Mfsd2a was either over-expressed or downregulated in the brain by administering Mfsd2a CRISPR activation or knockout plasmids, respectively. The potential mechanism of Mfsd2a-mediated BBB protection through the cav-1/Nrf-2/HO-1 signaling pathway was evaluated. Results: Mfsd2a levels were significantly decreased while cav-1, Nrf-2 and HO-1 levels were increased in the right frontal perisurgical area following SBI. When overexpressed, Mfsd2a attenuated brain edema and abolished neurologic impairment caused by SBI while downregulation of Mfsd2a expression further deteriorated BBB functions and worsened neurologic performance following SBI. The beneficial effect of Mfsd2a overexpression on BBB functions was associated with diminished expression of cav-1, increased Keap-1/Nrf-2 dissociation and further augmented levels of Nrf-2 and HO-1 in the right frontal perisurgical area, leading to enhanced levels of tight junction proteins following SBI. The BBB protective effect of Mfsd2a was blocked by selective inhibitors of Nrf-2 and HO-1. Conclusions: Mfsd2a attenuates BBB disruption through cav-1/Nrf-2/HO-1 signaling pathway in rats subjected to experimental SBI.

引用

页数：15