Evaluation of Phytochemical, Total phenolic and Antioxidant Activity of Carica Papaya Seed for Its Use in Biosynthesis of Gold Nanoparticles

Synthesis of nanoparticles using green methods with study of their propertie is one of great researchers' concerns in fields of pharmaceutical and biomedical products. The present paper aiming to use of an eco-friendly route is used for biosynthesis of gold nanoparticles (AuNPs) using C. Papaya seeds as both reducing and stabilizing agent. Study of C. Papaya seed contents showed many compounds which analyzed via qualitative, quantitative and GC-MS analysis. The influence of C. Papaya concentration, contact time and temperature on the reaction rate and shape of AuNPs are confirmed The biosynthesized NPs are characterize via UV-Vis, AFM and SEM. The AFM and SEM analysis exhibit a size between 40-105nm with average diameter 75.68nm, and spherical in structure. The antioxidant activity and free radical scavenge activity are studied by using TLC and DPPH analysis. The results show that the C. Papaya seeds and AuNPs have potent antioxidant activity; moreover, the total phenolic contents are characterized. The data confirmed that the C. Papaya seed extract was a good bio-reductant way for AuNPs that can be applied as a promising field in different bio-applications.