Highly sensitive chemiluminescent sensing of intracellular Al3+ based on the phosphatase mimetic activity of cerium oxide nanoparticles

被引：54

作者：

Tian, Xue ^{[1
]}

Liao, Hong ^{[1
]}

Wang, Min ^{[2
]}

Feng, Lingyan ^{[3
]}

Fu, Wensheng ^{[1
]}

Hu, Lianzhe ^{[1
]}

机构：

[1] Chongqing Normal Univ, Coll Chem, Chongqing Key Lab Green Synth & Applicat, Chongqing 401331, Peoples R China

[2] Chongqing Univ, Sch Pharmaceut Sci, Chongqing 401331, Peoples R China

[3] Shanghai Univ, Mat Genome Inst, Shanghai 200444, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2020年 / 152卷

基金：

中国国家自然科学基金;

关键词：

chemiluminescence; Nanozyme; Phosphatase; Biocatalyst; Enzyme mimic; PEROXIDASE-LIKE ACTIVITY; ALUMINUM; CHEMOSENSOR; NANOZYMES; NANOMATERIALS; FLUORESCENCE; SPECIATION; NANOCERIA; ENZYMES; LUMINOL;

D O I：

10.1016/j.bios.2020.112027

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Nanomaterials with enzyme-like characteristics (also called nanozymes) have attracted increasing attention in the area of analytical chemistry. Nevertheless, most of the nanozymes used for analytical applications are oxidoreductase mimics, and their enzyme-like activities are usually demonstrated by using chromogenic and/or fluorogenic substrates. Herein, the phosphatase mimetic activity of cerium oxide nanoparticles (nanoceria) was investigated by using CDP-star as the chemiluminescent (CL) substrate. Interestingly, we found that the phosphatase mimetic activity of nanoceria can be remarkably inhibited by the addition of Al3+ . Based on this finding, a highly sensitive and selective CL method for Al3+ detection is proposed. The CL intensity of the nanoceria/CDP-star system decreased with the increasing Al3+ concentrations in the range from 30 nM to 3.5 mu M. A detection limit as low as 10 mu M was obtained. Finally, the CL detection of intracellular Al3+ was achieved, demonstrating the utility of the CL method in complex biological samples.

引用

页数：7