In today's date, not one day goes by without the word 'stress'. Stress is the body's response when subjected to a stimulus, initiating a 'fight or flight'. Stress is a physical, mental, emotional factor, causing tension. There are innumerable signs, symptoms, causes and disorders that are stress-related, especially the post traumatic stress disorder, the acute stress disorder and comparatively higher mortality and morbidity rates, upto 50%. Thus, means to measure stress must be developed in a quick, cost effective manner. The current research deals with a sensor based biological method of stress measurement, performed by detecting the levels of biomarkers for stress - salivary amylase assay usinga chromogenic substrate, measurable at 405nm in biosensor equipments, miniature spectrophotometers and light resistance detectors - and correlating it to the normal unstressed levels of the enzyme biomarker present in the individual. (C) 2018 Elsevier Ltd. All rights reserved.