OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria

被引：39

作者：

Sluchanko, Nikolai N. ^{[1
,2
]}

Slonimskiy, Yury B. ^{[1
,3
]}

Shirshin, Evgeny A. ^{[4
]}

Moldenhauer, Marcus ^{[5
]}

Friedrich, Thomas ^{[5
]}

Maksimov, Eugene G. ^{[2
]}

机构：

[1] Russian Acad Sci, AN Bach Inst Biochem, Fed Res Ctr Biotechnol, Leninskiy Prospect 33,Bldg 1, Moscow 119071, Russia

[2] Moscow MV Lomonosov State Univ, Dept Biophys, Fac Biol, Leninskie Gory 1,Bldg 24, Moscow 119234, Russia

[3] Moscow MV Lomonosov State Univ, Dept Biochem, Fac Biol, Leninskie Gory 1,Bldg 12, Moscow 119234, Russia

[4] Moscow MV Lomonosov State Univ, Dept Quantum Elect, Fac Phys, Leninskie Gory 1,Bldg 62, Moscow 119991, Russia

[5] Tech Univ Berlin, Inst Chem PC 14, Str 17 Juni 135, D-10623 Berlin, Germany

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

俄罗斯基础研究基金会; 俄罗斯科学基金会;

关键词：

ORANGE CAROTENOID PROTEIN; FLUORESCENCE RECOVERY PROTEIN; SMALL-ANGLE SCATTERING; RAY SOLUTION SCATTERING; SYNECHOCYSTIS PCC 6803; PHOTOPROTECTIVE MECHANISM; QUENCHING MECHANISMS; MOLECULAR-MECHANISM; CRYSTAL-STRUCTURE; SIGNALING STATE;

D O I：

10.1038/s41467-018-06195-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In cyanobacteria, high light photoactivates the orange carotenoid protein (OCP) that binds to antennae complexes, dissipating energy and preventing the destruction of the photosynthetic apparatus. At low light, OCP is efficiently deactivated by a poorly understood action of the dimeric fluorescence recovery protein (FRP). Here, we engineer FRP variants with defined oligomeric states and scrutinize their functional interaction with OCP. Complemented by disulfide trapping and chemical crosslinking, structural analysis in solution reveals the topology of metastable complexes of OCP and the FRP scaffold with different stoichiometries. Unable to tightly bind monomeric FRP, photoactivated OCP recruits dimeric FRP, which subsequently monomerizes giving 1: 1 complexes. This could be facilitated by a transient OCP-2FRP-OCP complex formed via the two FRP head domains, significantly improving FRP efficiency at elevated OCP levels. By identifying key molecular interfaces, our findings may inspire the design of optically triggered systems transducing light signals into protein-protein interactions.

引用

页数：15

共 67 条

[41] RELAXING PROTEIN SYSTEM OF STRIATED MUSCLE - RESOLUTION OF TROPONIN COMPLEX INTO INHIBITORY AND CALCIUM ION-SENSITIZING FACTORS AND THEIR RELATIONSHIP TO TROPOMYOSIN [J].