Flipping interferometry and its application for quantitative phase microscopy in a micro-channel

被引：70

作者：

Roitshtain, Darina ^{[1
]}

Turko, Nir A. ^{[1
]}

Javidi, Bahram ^{[2
]}

Shaked, Natan T. ^{[1
]}

机构：

[1] Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel

[2] Univ Connecticut, Dept Elect & Comp Engn, Storrs, CT 06269 USA

来源：

OPTICS LETTERS | 2016年 / 41卷 / 10期

基金：

美国国家科学基金会;

关键词：

COMPACT;

D O I：

10.1364/OL.41.002354

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a portable, off-axis interferometric module for quantitative phase microscopy of live cells, positioned at the exit port of a coherently illuminated inverted microscope. The module creates on the digital camera an interference pattern between the image of the sample and its flipped version. The proposed simplified module is based on a retro-reflector modification in an external Michelson interferometer. The module does not contain any lenses, pinholes, or gratings and its alignment is straightforward. Still, it allows full control of the off-axis angle and does not suffer from ghost images. As experimentally demonstrated, the module is useful for quantitative phase microscopy of live cells rapidly flowing in a micro-channel. (C) 2016 Optical Society of America

引用

页码：2354 / 2357

页数：4

共 16 条

[1] Common path interferometer based on the modified Michelson configuration using a reflective grating [J].

Bai, Hongyi ;

Shan, Mingguang ;

Zhong, Zhi ;

Guo, Lili ;

Zhang, Yabin .

OPTICS AND LASERS IN ENGINEERING, 2015, 75 :1-4

[2] Label-free cell cycle analysis for high-throughput imaging flow cytometry [J].

Blasi, Thomas ;

Hennig, Holger ;

Summers, Huw D. ;

Theis, Fabian J. ;

Cerveira, Joana ;

Patterson, James O. ;

Davies, Derek ;

Filby, Andrew ;

Carpenter, Anne E. ;

Rees, Paul .

NATURE COMMUNICATIONS, 2016, 7

[3] Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd's mirror [J].

Chhaniwal, Vani ;

Singh, Amardeep S. G. ;

Leitgeb, Rainer A. ;

Javidi, Bahram ;

Anand, Arun .

OPTICS LETTERS, 2012, 37 (24) :5127-5129

[4]

Ghiglia DC., 1998, 2 DIMENSIONAL PHASE

[5] Fast phase processing in off-axis holography using multiplexing with complex encoding and live-cell fluctuation map calculation in real-time [J].

Girshovitz, Pinhas ;

Shaked, Natan T. .

OPTICS EXPRESS, 2015, 23 (07) :8773-8787

[6] Compact and portable low-coherence interferometer with off-axis geometry for quantitative phase microscopy and nanoscopy [J].

Girshovitz, Pinhas ;

Shaked, Natan T. .

OPTICS EXPRESS, 2013, 21 (05) :5701-5714

[7] Self-reference quantitative phase microscopy for microfluidic devices [J].

Jang, Jaeduck ;

Bae, Chae Yun ;

Park, Je-Kyun ;

Ye, Jong Chul .

OPTICS LETTERS, 2010, 35 (04) :514-516

[8] Off-axis interferometer with adjustable fringe contrast based on polarization encoding [J].

Karepov, Sharon ;

Shaked, Natan T. ;

Ellenbogen, Tal .

OPTICS LETTERS, 2015, 40 (10) :2273-2276

[9] Simplified approach for quantitative digital holographic phase contrast imaging of living cells [J].

Kemper, Bjoern ;

Vollmer, Angelika ;

Rommel, Christina E. ;

Schnekenburger, Juergen ;

von Bally, Gert .

JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (02)

[10] Quantitative phase imaging unit [J].

Lee, KyeoReh ;

Park, YongKeun .

OPTICS LETTERS, 2014, 39 (12) :3630-3633

← 1 2 →