Expression and characterization of VP2 protein of human rotavirus a in a mammalian lung cell line

被引：1
|
作者：
Pourasgari, F. [1 ]
Ahmadian, S. [1 ]
Salmanian, A. H. [2 ]
机构：
[1] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran
[2] Natl Inst Genet Engn & Biotechnol, Tehran, Iran
来源：
ACTA VIROLOGICA | 2007年 / 51卷 / 04期
关键词：
A549 cell line; core-like particles; expression; Human rotavirus A; VP2; gene;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A system for the expression and characterization of VP2 protein of Human rotavir-us A, strain G3 was established in the mammalian lung cell line (A549) Expression of the recombinant VP2 (rVP2) protein was detected 48-72 hrs after transfection by Western blotting. The rVP2 protein expressed in A549 cells formed intracellular core-like particles (CLPs) 30 nm in diameter detected by electron microscopy (EM). These results showed that the A549 cells are suitable as efficient eukaryotic host for production of rVP2 protein.
引用
收藏
页码：261 / 264
页数：4
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