Expression and characterization of VP2 protein of human rotavirus a in a mammalian lung cell line

被引：1
作者：
Pourasgari, F. [1 ]
Ahmadian, S. [1 ]
Salmanian, A. H. [2 ]
机构：
[1] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran
[2] Natl Inst Genet Engn & Biotechnol, Tehran, Iran
来源：
ACTA VIROLOGICA | 2007年 / 51卷 / 04期
关键词：
A549 cell line; core-like particles; expression; Human rotavirus A; VP2; gene;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A system for the expression and characterization of VP2 protein of Human rotavir-us A, strain G3 was established in the mammalian lung cell line (A549) Expression of the recombinant VP2 (rVP2) protein was detected 48-72 hrs after transfection by Western blotting. The rVP2 protein expressed in A549 cells formed intracellular core-like particles (CLPs) 30 nm in diameter detected by electron microscopy (EM). These results showed that the A549 cells are suitable as efficient eukaryotic host for production of rVP2 protein.
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页码：261 / 264
页数：4
相关论文
共 23 条
[1]   Detection of Rotavirus from stool samples using a standardized immuno-PCR ("Imperacer") method with end-point and real-time detection [J].
Adler, M ;
Schulz, S ;
Fischer, R ;
Niemeyer, CM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 333 (04) :1289-1294
[2]   Morphological study of the role of calcium in the assembly of the rotavirus outer capsid protein VP7 [J].
Ahmadian, S ;
Shahrabadi, MS .
BIOTECHNIC & HISTOCHEMISTRY, 1999, 74 (05) :266-273
[3]   RABBIT MODEL OF ROTAVIRUS INFECTION [J].
CONNER, ME ;
ESTES, MK ;
GRAHAM, DY .
JOURNAL OF VIROLOGY, 1988, 62 (05) :1625-1633
[4]   Nasal immunization of mice with virus-like particles protects offspring against rotavirus diarrhea [J].
Coste, A ;
Sirard, JC ;
Johansen, K ;
Cohen, J ;
Kraehenbuhl, JP .
JOURNAL OF VIROLOGY, 2000, 74 (19) :8966-8971
[5]   Nasal immunisation with Salmonella typhimurium producing rotavirus VP2 and VP6 antigens stimulates specific antibody response in serum and milk but fails to protect offspring [J].
Coste, A ;
Cohen, J ;
Reinhardt, M ;
Kraehenbuhl, JP ;
Sirard, JC .
VACCINE, 2001, 19 (30) :4167-4174
[6]   CHARACTERIZATION OF VIRUS-LIKE PARTICLES PRODUCED BY THE EXPRESSION OF ROTAVIRUS CAPSID PROTEINS IN INSECT CELLS [J].
CRAWFORD, SE ;
LABBE, M ;
COHEN, J ;
BURROUGHS, MH ;
ZHOU, YJ ;
ESTES, MK .
JOURNAL OF VIROLOGY, 1994, 68 (09) :5945-5952
[7]   Recent advances in mucosal vaccines and adjuvants [J].
Eriksson, K ;
Holmgren, J .
CURRENT OPINION IN IMMUNOLOGY, 2002, 14 (05) :666-672
[8]   NUCLEOTIDE-SEQUENCE OF GENOMIC SEGMENT-2 OF THE HUMAN ROTAVIRUS-WA [J].
ERNST, H ;
DUHL, JA .
NUCLEIC ACIDS RESEARCH, 1989, 17 (11) :4382-4382
[9]   ROTAVIRUS GENE STRUCTURE AND FUNCTION [J].
ESTES, MK ;
COHEN, J .
MICROBIOLOGICAL REVIEWS, 1989, 53 (04) :410-449
[10]   CHARACTERIZATION OF ROTAVIRUS REPLICATION INTERMEDIATES - A MODEL FOR THE ASSEMBLY OF SINGLE-SHELLED PARTICLES [J].
GALLEGOS, CO ;
PATTON, JT .
VIROLOGY, 1989, 172 (02) :616-627
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