Coating Electrospun Chitosan Nanofibers with Polyelectrolyte Multilayers Using the Polysaccharides Heparin and N,N,N-Trimethyl Chitosan

被引：46

作者：

Almodovar, Jorge ^{[1
]}

Kipper, Matt J. ^{[1
,2
]}

机构：

[1] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Sch Biomed Engn, Ft Collins, CO 80523 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 01期

基金：

美国国家科学基金会;

关键词：

biomaterials; fibroblast growth factor-2; glycosaminoglycan; layer-by-layer; polyelectrolytes; FIBROUS MEMBRANES; FIBERS; COLLAGEN;

D O I：

10.1002/mabi.201000261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method is presented for functionalizing electrospun nanofibers with GAGs and growth factors by PEM deposition. Electrospun chitosan nanofibers, spun from trifluoroacetic acid and dichloromethane, were coated with PEMs, using the polysaccharides heparin and N,N,N-trimethyl chitosan. FGF-2 was adsorbed on the PEM-coated nanofibers. Nanofiber neutralization, PEM construction, and FGF-2 adsorption were monitored using FT-IR spectroscopy and X-ray photoelectron spectroscopy. Alcian blue staining was used to confirm the presence of heparin. SEM was used to study nanofiber morphology.

引用

收藏

页码：72 / 76

页数：5

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