Cholestane and spirostane-type glycosides from the roots and rhizomes of Panicum repens L.

Chemical investigation of the ethanolic extract from roots and rhizomes of Panicum repens L. has led to the isolation of four new steroidal glycosides, (25S)-spirost-5-en-3 beta-ol-3-O-alpha-L-rhamnopyranosyl(1 -> 4)-O-alpha-L-rhamnopyranosyl-(1 -> 4)-O-alpha-L-rhamnopyranosyl-(1 -> 2)-O-beta-D-glucopyranoside (1); 16-O-alpha-L-rhamnopyranosyl-cholest-5-en-2 alpha, 3 beta, 16 beta-triol-22-one-3-O-alpha-L-rhamnopyranoside (2); 16O-alpha-L-rhamnopyranosyl-cholest-5-en-3 beta, 16 beta diol-2 alpha-methoxy-22-one-3-O-alpha-L-rhamnopyranoside (3) and 16-O-alpha-L-rhamnopyranosyl-cholest-5-en-3 beta, 16 beta-diol-2 alpha-methoxy-3-O-alpha-L-rhamnopyranoside (4). Their structures were elucidated by means of extensive NMR spectral studies. Five known spirostane glycosides were also isolated and characterized by comparison with the literature data. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.