Use of Span 80 reverse micellar system for the extraction of pectinesterase from lemon peel waste

被引：1
作者：
Peng, Xin [1 ,2 ]
Du, Hao [1 ]
Xu, Peng-Fei [1 ]
Tang, Yu [1 ]
Meng, Yong [1 ]
Yuan, Lu [1 ]
机构：
[1] Hunan Normal Univ, Hunan Normal Univ,Coll Chem, Natl & Local Joint Engn Lab New Petro, Key Lab Chem Biol,Key Lab Assembly, Changsha, Peoples R China
[2] Hunan Normal Univ, Hunan Normal Univ,Coll Chem,Coll Chem, Natl & Local Joint Engn Lab New Petro, Key Lab Chem Biol,Key Lab Assembly, Changsha, Peoples R China
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2020年 / 55卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Reverse micelle; Span; 80; pectinesterase; activity recovery; backward extraction efficiency; PINEAPPLE PEEL; PURIFICATION; TEMPERATURE; NATTOKINASE; BROMELAIN; LACCASE; LECTIN; PHASE; AOT;
D O I：
10.1080/01496395.2019.1588318
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Reverse micellar systems of nonionic surfactant Span 80/isooctane/n-hexane is used for the extraction of pectinesterase from crude aqueous extract of lemon peel waste. The effect of extraction process parameters on the extraction efficiency and activity recovery were studied in detail for the lemon peel extract. The optimum NaCl concentration (0.2 mol/L), Span 80 concentration (0.3 mol/L), the extraction phase pH (7.0), the temperature (60 degrees C), and oscillation time (30 min) were found out and the optimized conditions for the extraction from lemon peel waste resulted in back extraction efficiencies of 52.4%. A fairly good activity recovery (83.7%) of pectinesterase is obtained under these conditions.
引用
收藏
页码：1115 / 1122
页数：8
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