Use of Span 80 reverse micellar system for the extraction of pectinesterase from lemon peel waste

被引：1
作者：
Peng, Xin [1 ,2 ]
Du, Hao [1 ]
Xu, Peng-Fei [1 ]
Tang, Yu [1 ]
Meng, Yong [1 ]
Yuan, Lu [1 ]
机构：
[1] Hunan Normal Univ, Hunan Normal Univ,Coll Chem, Natl & Local Joint Engn Lab New Petro, Key Lab Chem Biol,Key Lab Assembly, Changsha, Peoples R China
[2] Hunan Normal Univ, Hunan Normal Univ,Coll Chem,Coll Chem, Natl & Local Joint Engn Lab New Petro, Key Lab Chem Biol,Key Lab Assembly, Changsha, Peoples R China
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2020年 / 55卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Reverse micelle; Span; 80; pectinesterase; activity recovery; backward extraction efficiency; PINEAPPLE PEEL; PURIFICATION; TEMPERATURE; NATTOKINASE; BROMELAIN; LACCASE; LECTIN; PHASE; AOT;
D O I：
10.1080/01496395.2019.1588318
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Reverse micellar systems of nonionic surfactant Span 80/isooctane/n-hexane is used for the extraction of pectinesterase from crude aqueous extract of lemon peel waste. The effect of extraction process parameters on the extraction efficiency and activity recovery were studied in detail for the lemon peel extract. The optimum NaCl concentration (0.2 mol/L), Span 80 concentration (0.3 mol/L), the extraction phase pH (7.0), the temperature (60 degrees C), and oscillation time (30 min) were found out and the optimized conditions for the extraction from lemon peel waste resulted in back extraction efficiencies of 52.4%. A fairly good activity recovery (83.7%) of pectinesterase is obtained under these conditions.
引用
收藏
页码：1115 / 1122
页数：8
相关论文
共 50 条
  • [1] Reverse micellar extraction of bromelain from pineapple peel - Effect of surfactant structure
    Wan, Jing
    Guo, Jingjing
    Miao, Zhitong
    Guo, Xia
    FOOD CHEMISTRY, 2016, 197 : 450 - 456
  • [2] Extraction of peroxidase from apple peel waste mediated by tween 80 reverse micelle system
    Chen, Xiangyan
    Fei, Jia
    Peng, Xin
    Meng, Yong
    Yuan, Lu
    2020 INTERNATIONAL CONFERENCE ON GREEN DEVELOPMENT AND ENVIRONMENTAL SCIENCE AND TECHNOLOGY, 2020, 615
  • [3] Waste Frying Oil Hydrolysis in a Reverse Micellar System
    Moya-Ramirez, Ignacio
    Garcia-Roman, Miguel
    Fernandez-Arteaga, Alejandro
    ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2016, 4 (03): : 1025 - 1031
  • [4] Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes
    Hebbar, H. Umesh
    Sumana, B.
    Raghavarao, K. S. M. S.
    BIORESOURCE TECHNOLOGY, 2008, 99 (11) : 4896 - 4902
  • [5] A Metal-Chelate Affinity Reverse Micellar System for Protein Extraction
    Dong, Xiao-Yan
    Meng, Yao
    Feng, Xu-Dong
    Sun, Yan
    BIOTECHNOLOGY PROGRESS, 2010, 26 (01) : 150 - 158
  • [6] Affinity based reverse micellar extraction and purification of bromelain from pineapple (Ananas comosus L. Merryl) waste
    Kumar, Sunil
    Hemavathi, A. B.
    Hebbar, H. Umesh
    PROCESS BIOCHEMISTRY, 2011, 46 (05) : 1216 - 1220
  • [7] Reverse Micellar Extraction of Copper Ions from Wastewater: Modelling and Simulation
    Chaturvedi, Tarun Kumar
    Pandit, Prabhat
    Upadhyaya, Sushant
    Vashishtha, Manish
    ASIAN JOURNAL OF WATER ENVIRONMENT AND POLLUTION, 2022, 19 (01) : 9 - 16
  • [8] Reverse micellar extraction of papain with cationic detergent based system: An optimization approach
    Prabhu, Ashish A.
    Chityala, Sushma
    Garg, Yachna
    Dasu, V. Venkata
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2017, 47 (03) : 236 - 244
  • [9] Reverse micellar extraction of lectin from black turtle bean (Phaseolus vulgaris): Optimisation of extraction conditions by response surface methodology
    He, Shudong
    Shi, John
    Walid, Elfalleh
    Zhang, Hongwei
    Ma, Ying
    Xue, Sophia Jun
    FOOD CHEMISTRY, 2015, 166 : 93 - 100
  • [10] Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system
    Pawar, Swapnali S.
    Iyyaswami, Regupathi
    Belur, Prasanna D.
    JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2017, 54 (11): : 3630 - 3639
12345