Neferine from Nelumbo nucifera modulates oxidative stress and cytokines production during hypoxia in human peripheral blood mononuclear cells

被引：19

作者：

Baskaran, Rathinasamy ^{[1
,2
,6
]}

Priya, Lohanathan Bharathi ^{[2
]}

Kalaiselvi, Palanisamy ^{[2
]}

Poornima, Paramasivan ^{[2
]}

Huang, Chih-Yang ^{[3
,4
]}

Padma, Viswanadha Vijaya ^{[1
,2
,3
,5
]}

机构：

[1] Bharathiar Univ, DRDO BU Ctr Life Sci, Coimbatore 641046, Tamil Nadu, India

[2] Bharathiar Univ, Dept Biotechnol, Translat Res Lab, Coimbatore 641046, Tamil Nadu, India

[3] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan

[4] China Med Univ, Sch Chinese Med, Taichung, Taiwan

[5] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan

[6] Natl Hlth Res Inst, Natl Inst Canc Res, Zhunan 35053, Miaoli County, Taiwan

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2017年 / 93卷

关键词：

Neferine; Hypoxia; Inflammation; Oxidative stress; IL-6; HYPOBARIC HYPOXIA; HIGH-ALTITUDE; SEED EMBRYO; EXPRESSION; GLUTATHIONE; APOPTOSIS; PATHWAY; INTERLEUKIN-8; MACROPHAGES; ACTIVATION;

D O I：

10.1016/j.biopha.2017.07.003

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Neferine, an alkaloid from N. nucifera has a broad range of pharmacological activity. Hypoxia mediated stress is involved in the generation of inflammatory responses and cell death. The present study evaluated the protective effect of neferine against hypoxia-induced cytotoxicity, oxidative stress and inflammatory response in human Peripheral Blood Mononuclear Cells (hPBMC). Cytotoxicity, as determined by MTT, LDH and NO assays revealed that 24 h of hypoxic exposure results in 20% cell death (IC20) and compromising of cellular integrity, which was restored to near control values by pretreatment with neferine. Oxidative stress parameters such as lipid peroxidation and antioxidant enzymes indicated that neferine exerted a protective effect on hypoxia-induced oxidative stress. Hypoxia-induced Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin 6 (IL-6), and Interleukin 8 (IL-8) release were significantly reduced in the neferine pretreated samples indicating its anti-inflammatory role. Our results demonstrate for the first time that neferine exerts a cytoprotective effect against hypoxia-induced oxidative stress and inflammation in hPBMC. (C) 2017 Elsevier Masson SAS. All rights reserved.

引用

页码：730 / 736

页数：7

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