Validation and application of a method for the simultaneous absolute quantification of 16 neutral and acidic human milk oligosaccharides by graphitized carbon liquid chromatography - electrospray ionization - mass spectrometry

被引：64
作者：
Tonon, Karina M. [1 ]
Miranda, Antonio [2 ]
Abrao, Ana Cristina F., V [3 ]
de Morais, Mauro B. [4 ]
Morais, Tania B. [5 ]
机构：
[1] Univ Fed Sao Paulo, Nutr Postgrad Program, Rua Napoleao de Barros, Sao Paulo, SP, Brazil
[2] Univ Fed Sao Paulo, Dept Biophys, Rua Tres de Maio, Sao Paulo, SP, Brazil
[3] Univ Fed Sao Paulo, Breastfeeding Incent & Support Ctr, Rua Diogo de Faria, Sao Paulo, SP, Brazil
[4] Univ Fed Sao Paulo, Div Pediat Gastroenterol, Rua Coronel Lisboa, Sao Paulo, SP, Brazil
[5] Univ Fed Sao Paulo, Food Qual Control Lab, Nutr Postgrad Program, Rua Napoleao de Barros, Sao Paulo, SP, Brazil
来源：
FOOD CHEMISTRY | 2019年 / 274卷
基金：
巴西圣保罗研究基金会;
关键词：
Human milk oligosaccharides; Sample preparation; Porous graphitic carbon; LC-ESI-MS; Secretor phenotype; Lewis phenotype; BREAST-FED INFANTS; BOVINE-MILK; LACTATION; 2-AMINOACRIDONE; QUANTITATION; CONSUMPTION; MICROBIOTA; COLOSTRUM; FECES; WOMEN;
D O I：
10.1016/j.foodchem.2018.09.036
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Human milk oligosaccharides (HMOs) are free glycans naturally present in human milk that act as prebiotics, prevent pathogen binding, modulate the immune system and support brain development in infants. The HMOs composition and concentrations vary significantly among different women mainly because of the direct influence of the Secretor and Lewis phenotypes on HMOs biosynthesis. Analytical methods that can identify the differences in the HMOs composition and concentrations are a fundamental tool in HMOs research. This paper describes a simple HMOs extraction and analysis for the simultaneous and absolute quantification of neutral and acidic HMOs by graphitized carbon liquid chromatography-electrospray ionization-mass spectrometry. This method was validated and applied to analyze HMOs in the human milk obtained from 10 women. This method allows accurate and reliable quantification of HMOs and can be used to determine differences in HMOs concentrations throughout lactation and among women with different Secretor and Lewis phenotypes.
引用
收藏
页码：691 / 697
页数：7
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