The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A

被引：9

作者：

Tsai, Meng-Lin ^{[1
]}

Cronin, Nora ^{[2
]}

Djordjevic, Snezana ^{[1
]}

机构：

[1] UCL, Div Biosci, Inst Struct & Mol Biol, London WC1E 6BT, England

[2] Univ London, Birkbeck Coll, Inst Struct & Mol Biol, London WC1E 7HX, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2011年 / 67卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

signal transduction; protein phosphatase 2A; methyltransferases; methylation; protein phosphatase methylesterases; CATALYTIC SUBUNIT; ENDOTHELIAL-CELLS; CRYSTAL-STRUCTURE; 2A; KINASE; DEPHOSPHORYLATION; PHOSPHORYLATION; INACTIVATION; METHYLATION; ACTIVATION;

D O I：

10.1107/S0907444910042204

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). PP2A, a key regulator of many cellular processes, has recently generated additional interest as a potential cancer-therapeutic target. The status of PP2A methylation impacts upon the selection of the regulatory subunit by the PP2A core enzyme, thus directing its activity and subcellular localization. An X-ray crystal structure of human LCMT1 protein in complex with the cofactor S-adenosylmethionine (AdoMet) has been solved to a resolution of 2 A. The structure enables the postulation of a mode of interaction with protein phosphatase PP2A and provides a platform for further functional studies of the regulation of methylation of PP2A.

引用

页码：14 / 24

页数：11

共 73 条

[1] Protein phosphatase 2A regulates apoptosis in neutrophils by dephosphorylating both p38 MAPK and its substrate caspase 3
Alvarado-Kristensson, M
Andersson, T
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (07) : 6238 - 6244
[2] REQUIREMENT FOR INTEGRATION OF SIGNALS FROM 2 DISTINCT PHOSPHORYLATION PATHWAYS FOR ACTIVATION OF MAP KINASE
ANDERSON, NG
MALLER, JL
TONKS, NK
STURGILL, TW
[J]. NATURE, 1990, 343 (6259) : 651 - 653
[3] The catalytic subunit of protein phosphatase 2A associates with the translation termination factor eRF1
Andjelkovic, N
Zolnierowicz, S
VanHoof, C
Goris, J
Hemmings, BA
[J]. EMBO JOURNAL, 1996, 15 (24) : 7156 - 7167
[4] HUMAN-LIVER PHOSPHATASE 2A - CDNA AND AMINO-ACID SEQUENCE OF 2 CATALYTIC SUBUNIT ISOTYPES
ARINO, J
CHEE, WW
BRAUTIGAN, DL
MILLER, TB
JOHNSON, GL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) : 4252 - 4256
[5] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY
BAILEY, S
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 : 760 - 763
[6] cAMP counter-regulates insulin-mediated protein phosphatase-2A inactivation in rat skeletal muscle cells
Begum, N
Ragolia, L
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (49) : 31166 - 31171
[7] Role of Janus kinase-2 in insulin-mediated phosphorylation and inactivation of protein phosphatase-2A and its impact on upstream insulin signalling components
Begum, N
Ragolia, L
[J]. BIOCHEMICAL JOURNAL, 1999, 344 : 895 - 901
[8] The Protein Data Bank
Berman, HM
Westbrook, J
Feng, Z
Gilliland, G
Bhat, TN
Weissig, H
Shindyalov, IN
Bourne, PE
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (01) : 235 - 242
[9] Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Bα subunit
Bryant, JC
Westphal, RS
Wadzinski, BE
[J]. BIOCHEMICAL JOURNAL, 1999, 339 : 241 - 246
[10] Protein structure prediction servers at university college london
Bryson, K
McGuffin, LJ
Marsden, RL
Ward, JJ
Sodhi, JS
Jones, DT
[J]. NUCLEIC ACIDS RESEARCH, 2005, 33 : W36 - W38

← 1 2 3 4 5 6 7 8 →