Identification of microRNA differentially expressed in three subtypes of non-small cell lung cancer and in silico functional analysis

被引:20
作者
Hu, Yanjun [1 ]
Wang, Luqing [2 ]
Gu, Jingxian [3 ]
Qu, Kai [3 ]
Wang, Yunxia [4 ]
机构
[1] Taishan Med Coll, Liaocheng Peoples Hosp, Dept Clin Lab, Liaocheng 252000, Shandong, Peoples R China
[2] Taishan Med Coll, Dept Nucl Med, Liaocheng Peoples Hosp, Radioimmunol Room, Liaocheng 252000, Shandong, Peoples R China
[3] Xi An Jiao Tong Univ, Dept Hepatobiliary Surg, Affiliated Hosp 1, Xian 710061, Shaanxi, Peoples R China
[4] Liaocheng Infect Dis Hosp, Dept Intens Care Unit, Liaocheng 252000, Shandong, Peoples R China
基金
美国国家科学基金会;
关键词
non-small cell lung cancer; histological subtype; differentially expressed miRNAs; HIPPO PATHWAY; ADENOCARCINOMA; CARCINOMA; SIGNATURES; HISTOLOGY; SURVIVAL; PROGNOSIS; SMOKING; MIR-375; ZO-1;
D O I
10.18632/oncotarget.20218
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Emerging studies demonstrated that miRNAs played fundamental roles in lung cancer. In this study, we attempted to explore the clinical significance of the miRNA signature in different histological subtypes of non-small cell lung cancer (NSCLC). Three miRNome profiling datasets (GSE19945, GSE25508 and GSE51853) containing lung squamous cell carcinoma (SCC), lung adenocarcinoma (ADC) and large cell lung cancer (LCLC) samples were obtained for bioinformatics and survival analysis. Moreover, pathway enrichment and coexpression network were performed to explore underlying molecular mechanism. MicroRNA-375 (miR-375), miR-203 and miR-205 were identified as differentially expressed miRNAs (DEmiRNAs) which distinguished SCC from other NSCLC subtypes. Pathway enrichment analysis suggested that Hippo signaling pathway was combinatorically affected by above mentioned three miRNAs. Coexpression analysis of three miRNAs and the Hippo signaling pathway related genes were conducted based on another dataset, GSE51852. Four hub genes (TP63, RERE, TJP1 and YWHAE) were identified as the candidate targets of three miRNAs, and three of them (TP63, TJP1 and YWHAE) were validated to be downregulated by miR-203 and miR-375, respectively. Finally, survival analysis further suggested the prognostic value of three-miRNA signature in SCC patients. Taken together, our study compared the miRNA profiles among three histological subtypes of NSCLC, and suggested that a three-miRNA signature might be potential diagnostic and prognostic biomarkers for SCC patients.
引用
收藏
页码:74554 / 74566
页数:13
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