Multivariate optimization of a method for the determination of fatty acids in dental biofilm by GC-MS

被引：5

作者：

Roth, Simon R. ^{[1
]}

Henkel, Kerstin ^{[1
,2
]}

Altenburger, Markus J. ^{[3
]}

Auwaerter, Volker ^{[1
]}

Neukamm, Merja A. ^{[1
]}

机构：

[1] Univ Freiburg, Med Ctr, Inst Forens Med, Fac Med, Albertstr 9, D-79104 Freiburg, Germany

[2] Univ Freiburg, Hermann Staudinger Grad Sch, Hebelstasse 27, D-79104 Freiburg, Germany

[3] Univ Freiburg, Med Ctr, Dept Operat Dent & Periodontol, Ctr Dent Med,Fac Med, Hugstetter Str 55, D-79106 Freiburg, Germany

来源：

BIOANALYSIS | 2018年 / 10卷 / 16期

关键词：

dental plaque; fatty acids; lipids; method development; toxicology; transesterification; validation; GRAM-NEGATIVE BACTERIA; LIQUID-CHROMATOGRAPHY; MICROBIAL COMMUNITIES; STREPTOCOCCUS-MUTANS; MASS-SPECTROMETRY; SOIL; BIOMASS; PLAQUE; ADAPTATION; PELLICLE;

D O I：

10.4155/bio-2018-0106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: Phospholipid fatty acid methyl ester (FAME) analysis offers a simple option additionally to 16S rRNA sequencing to characterize microbial communities and to monitor changes. A method was established for the characterization of dental plaque via FAME profiles. Methodology: Fatty acids were determined as FAMEs (direct, acidic transesterification) and analyzed by GC-MS using an optimized temperature gradient. The transesterification reaction was optimized using a fractional factorial central composite face-centered design. Results: Optimal conditions for the transesterification in methanol/toluene: hydrochloric acid concentration 2% (w/v), reaction time 40 min, temperature 110 degrees C. Method validation showed satisfactory accuracy, precision and linearity. Conclusion: The method provides a useful tool to characterize plaque via FAME profiles and was successfully applied to samples from ten subjects demonstrating its applicability.

引用

页码：1319 / 1333

页数：15

共 30 条

[1] Fatty acid profile of human saliva:: a possible indicator of dietary fat intake [J].

Actis, AB ;

Perovic, NR ;

Defagó, D ;

Beccacece, C ;

Eynard, AR .

ARCHIVES OF ORAL BIOLOGY, 2005, 50 (01) :1-6

[2] Streptococcus mutans membrane lipid composition: Virulence factors and structural parameters [J].

Bojanich, M. A. ;

Calderon, R. O. .

ARCHIVES OF ORAL BIOLOGY, 2017, 81 :74-80

[3] Rapid adaptation of Rhodococcus erythropolis cells to salt stress by synthesizing polyunsaturated fatty acids [J].

de Carvalho, Carla C. C. R. ;

Marques, Marco P. C. ;

Hachicho, Nancy ;

Heipieper, Hermann J. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (12) :5599-5606

[4] The Human Oral Microbiome [J].

Dewhirst, Floyd E. ;

Chen, Tuste ;

Izard, Jacques ;

Paster, Bruce J. ;

Tanner, Anne C. R. ;

Yu, Wen-Han ;

Lakshmanan, Abirami ;

Wade, William G. .

JOURNAL OF BACTERIOLOGY, 2010, 192 (19) :5002-5017

[5]

Frostegard A, 1996, BIOL FERT SOILS, V22, P59, DOI 10.1007/BF00384433

[6]

FROSTEGARD A, 1993, APPL ENVIRON MICROB, V59, P3605

[7] Use and misuse of PLFA measurements in soils [J].

Frostegard, Asa ;

Tunlid, Anders ;

Baath, Erland .

SOIL BIOLOGY & BIOCHEMISTRY, 2011, 43 (08) :1621-1625

[8]

German Institute for Standardization, 2017, 3840251201705 DIN GE

[9]

Hachicho N, 2016, HYDROCARBON LIPID MI, P123

[10] Full validation of a method for the determination of drugs of abuse in non-mineralized dental biofilm using liquid chromatography-tandem mass spectrometry and application to postmortem samples [J].

Henkel, Kerstin ;

Altenburger, Markus J. ;

Auwaerter, Volker ;

Neukamm, Merja A. .

TALANTA, 2018, 176 :360-366

← 1 2 3 →