Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells

被引：107
作者：
Cvorovic, Jovana [1 ]
Tramer, Federica [1 ]
Granzotto, Marilena [2 ]
Candussio, Luigi [1 ]
Decorti, Giuliana [1 ]
Passamonti, Sabina [1 ]
机构：
[1] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy
[2] IRCCS Burlo Garofolo, Immunol Lab, I-34137 Trieste, Italy
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2010年 / 501卷 / 01期
关键词：
Anthocyanidins; Colon cancer cells; Cytotoxicity; Apoptosis; Oxidative stress; Glutathione; GLUTATHIONE; ANTHOCYANINS; ANTIOXIDANT; MECHANISMS; RESISTANCE; ASSAY; PROLIFERATION; INHIBITION; METABOLISM; MODULATION;
D O I：
10.1016/j.abb.2010.05.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Colorectal cancer is the second most frequent cause of cancer death in the western world. Although the prognosis has improved after the introduction of newer anticancer drugs, the treatment of metastatic colorectal cancer still remains a challenge due to a high percentage of drug-resistant tumor forms. We aimed at testing whether anthocyanidins exerted cytotoxicity in primary (Caco-2) and metastatic (LoVo and LoVo/ADR) colorectal cancer cell lines. Both cyanidin and delphinidin, though neither pelargonidin nor malvidin, were cytotoxic in metastatic cells only. The cell line most sensitive to anthocyanidins was the drug-resistant LoVo/ADR. There, cellular ROS accumulation, inhibition of glutathione reductase, and depletion of glutathione could be observed. This suggests that anthocyanidins may be used as sensitizing agents in metastatic colorectal cancer therapy. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：151 / 157
页数：7
相关论文
共 31 条
[1]   Sequential opening of mitochondrial ion channels as a function of glutathione redox thiol status [J].
Aon, Miguel A. ;
Cortassa, Sonia ;
Maack, Christoph ;
O'Rourke, Brian .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (30) :21889-21900
[2]   A Dietary Anthocyanidin Delphinidin Induces Apoptosis of Human Prostate Cancer PC3 Cells In vitro and In vivo: Involvement of Nuclear Factor-κB Signaling [J].
Bin Hafeez, Bilal ;
Siddiqui, Imtiaz Ahmad ;
Asim, Mohammad ;
Malik, Arshi ;
Afaq, Farrukh ;
Adhami, Vaqar Mustafa ;
Saleem, Mohammad ;
Din, Maria ;
Mukhtar, Hasan .
CANCER RESEARCH, 2008, 68 (20) :8564-8572
[3]   Radical scavenging capacity of wine anthocyanins is strongly pH-dependent [J].
Borkowski, T ;
Szymusiak, H ;
Gliszczynska-Swiglo, A ;
Rietjens, IMCM ;
Tyrakowska, B .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (14) :5526-5534
[4]   INDUCTION BY VERAPAMIL OF A RAPID INCREASE IN ATP CONSUMPTION IN MULTIDRUG-RESISTANT TUMOR-CELLS [J].
BROXTERMAN, HJ ;
PINEDO, HM ;
KUIPER, CM ;
KAPTEIN, LCM ;
SCHUURHUIS, GJ ;
LANKELMA, J .
FASEB JOURNAL, 1988, 2 (07) :2278-2282
[5]   The cytotoxicity of dopamine may be an artefact of cell culture [J].
Clement, MW ;
Long, LH ;
Ramalingam, J ;
Halliwell, B .
JOURNAL OF NEUROCHEMISTRY, 2002, 81 (03) :414-421
[6]   Transport of glutathione and glutathione conjugates by MRP1 [J].
Cole, Susan P. C. ;
Deeley, Roger G. .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2006, 27 (08) :438-446
[7]   Chemoresistant Colorectal Cancer Cells, the Cancer Stem Cell Phenotype, and Increased Sensitivity to Insulin-like Growth Factor-I Receptor Inhibition [J].
Dallas, Nikolaos A. ;
Xia, Ling ;
Fan, Fan ;
Gray, Michael J. ;
Gaur, Puja ;
van Buren, George, II ;
Samuel, Shaija ;
Kim, Michael P. ;
Lim, Sherry J. ;
Ellis, Lee M. .
CANCER RESEARCH, 2009, 69 (05) :1951-1957
[8]   INHIBITION OF GLUTATHIONE-REDUCTASE BY FLAVONOIDS - A STRUCTURE ACTIVITY STUDY [J].
ELLIOTT, AJ ;
SCHEIBER, SA ;
THOMAS, C ;
PARDINI, RS .
BIOCHEMICAL PHARMACOLOGY, 1992, 44 (08) :1603-1608
[9]  
Fanciulli M, 2000, CLIN CANCER RES, V6, P1590
[10]   Cyanidin-3-rutinoside, a natural polyphenol antioxidant, selectively kills leukemic cells by induction of oxidative stress [J].
Feng, Rentian ;
Ni, Hong-Min ;
Wang, Shiow Y. ;
Tourkova, Irina L. ;
Shurin, Michael R. ;
Harada, Hisashi ;
Yin, Xiao-Ming .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (18) :13468-13476
1234