Upregulation of miR-520b promotes ovarian cancer growth

被引：26

作者：

Guan, Rui ^{[1
]}

Cai, Shengyun ^{[1
]}

Sun, Mingjuan ^{[2
]}

Xu, Mingjuan ^{[1
]}

机构：

[1] Second Mil Med Univ, Changhai Hosp, Dept Obstet & Gynecol, 168 Changhai Rd, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China

来源：

ONCOLOGY LETTERS | 2017年 / 14卷 / 03期

关键词：

miR-520b; ovarian cancer; RNF216; cancer growth; HEPATOCELLULAR-CARCINOMA; MICRORNAS; BIOMARKERS; TARGET; SERUM; PCR;

D O I：

10.3892/ol.2017.6552

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Ovarian cancer is the most common gynecological malignant cancer in female genitalia. Dysregulation or dysfunction of microRNAs (miRs) contribute to cancer development. The role of miR-520b in ovarian cancer remains unclear. The present study investigated the role of miR-520b in ovarian cancer and determined that the expression levels of miR-520b in ovarian cancer tissues and cell lines were upregulated. By contrast, reverse transcription-quantitative polymerase chain reaction and immunohistochemistry revealed that the mRNA and protein expression levels of ring finger protein 216 (RNF216) were downregulated in ovarian cancer, indicating that there was a negative correlation between miR-520b and RNF216. In miR-520b-knockdown cells, downregulation of miR-520b reduced cell proliferation, while upregulation of miR-520b promoted cell proliferation. In addition, RNF216 was predicted by TargetScanHuman and was observed to be targeted by miR-520b. In conclusion, the present data indicated that high expression of miR-520b in ovarian cancer promoted cell growth via RNF216.

引用

页码：3155 / 3161

页数：7

共 33 条

[1] The functions of animal microRNAs [J].

Ambros, V .

NATURE, 2004, 431 (7006) :350-355

[2]

[Anonymous], COCHRANE DATABASE SY

[3] Survival effect of maximal cytoreductive surgery for advanced ovarian carcinoma during the platinum era: A meta-analysis [J].

Bristow, RE ;

Tomacruz, RS ;

Armstrong, DK ;

Trimble, EL ;

Montz, FJ .

JOURNAL OF CLINICAL ONCOLOGY, 2002, 20 (05) :1248-1259

[4] Detection of MicroRNA as Novel Biomarkers of Epithelial Ovarian Cancer From the Serum of Ovarian Cancer Patient [J].

Chung, Ye-Won ;

Bae, Hyo-Sook ;

Song, Jae-Yun ;

Lee, Jae Kwan ;

Lee, Nak Woo ;

Kim, Tak ;

Lee, Kyu-wan .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2013, 23 (04) :673-679

[5] Causes and consequences of microRNA dysregulation in cancer [J].

Croce, Carlo M. .

NATURE REVIEWS GENETICS, 2009, 10 (10) :704-714

[6] Most mammalian mRNAs are conserved targets of microRNAs [J].

Friedman, Robin C. ;

Farh, Kyle Kai-How ;

Burge, Christopher B. ;

Bartel, David P. .

GENOME RESEARCH, 2009, 19 (01) :92-105

[7] Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs [J].

Garcia, David M. ;

Baek, Daehyun ;

Shin, Chanseok ;

Bell, George W. ;

Grimson, Andrew ;

Bartel, David P. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2011, 18 (10) :1139-U75

[8] MicroRNAs in Cancer [J].

Lee, Yong Sun ;

Dutta, Anindya .

ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE, 2009, 4 :199-227

[9]

Grentzmann G, 1998, RNA, V4, P479

[10] MicroRNA targeting specificity in mammals: Determinants beyond seed pairing [J].

Grimson, Andrew ;

Farh, Kyle Kai-How ;

Johnston, Wendy K. ;

Garrett-Engele, Philip ;

Lim, Lee P. ;

Bartel, David P. .

MOLECULAR CELL, 2007, 27 (01) :91-105

← 1 2 3 4 →