Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging

被引：40

作者：

Rowlands, Christopher J. ^{[1
]}

Varma, Sandeep ^{[2
]}

Perkins, William ^{[2
]}

Leach, Iain ^{[3
]}

Williams, Hywel ^{[4
]}

Notingher, Ioan ^{[1
]}

机构：

[1] Univ Nottingham, Sch Phys & Astron, Nottingham NG7 2RD, England

[2] Nottingham Univ Hosp NHS Trust, Dept Dermatol, Nottingham NG7 2UH, England

[3] Nottingham Univ Hosp NHS Trust, Dept Histopathol, Nottingham NG7 2UH, England

[4] Nottingham Univ Hosp NHS Trust, Ctr Evidence Based Dermatol, Nottingham NG7 2UH, England

来源：

JOURNAL OF BIOPHOTONICS | 2012年 / 5卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Raman spectroscopy; image reconstruction; computer assisted diagnosis; microscopy; PRINCIPAL COMPONENT ANALYSIS; BASAL-CELL CARCINOMA; SCATTERING MICROSCOPY; SPECTROSCOPY; LINE;

D O I：

10.1002/jbio.201100098

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for acquiring high-spatial-resolution spectral maps, in particular for Raman micro-spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating the results. This method achieves up to 30 times reduction in the sampling time compared to raster-scanning, the resulting images have excellent correlation with conventional histopathological staining, and are achieved with sufficient spectral signal-to-noise ratio to identify individual tissue structures. The benefits of this selective sampling method are not limited to tissue imaging however; it is expected that the method may be applied to other techniques which employ point-by-point mapping of large substrates. (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

引用

页码：220 / 229

页数：10

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