Elastase from polymorphonuclear leukocyte in articular cartilage and synovial fluids of patients with rheumatoid arthritis

被引:41
作者
Momohara, S
Kashiwazaki, S
Inoue, K
Saito, S
Nakagawa, T
机构
[1] Department of Orthopaedic Surgery, Keio University, Tokyo
[2] Institute of Rheumatology, Tokyo Women's Medical College, Tokyo
[3] Institute of Rheumatology, Tokyo Women's Medical College, KS Building, Shinjuku-ku, Tokyo 162
关键词
rheumatoid arthritis; serine proteinases; PMN elastase; articular cartilage; stromelysin-1; tissue inhibitor of metalloproteinases-1 (TIMP-1);
D O I
10.1007/BF02247841
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective was to study the significance and the mechanism of action of elastase from polymorphonuclear leukocyte (PMN elastase) in patients with rheumatoid arthritis (RA), The experiments conducted consisted of two phases, Firstly, articular cartilage and synovia from 8 patients with RA undergoing total knee replacement were obtained, and the gelatinolytic enzyme activity was extracted with 2M guanidine hydrochloride. The gelatinolytic activity of each tissue was measured to confirm that the activity was due to PMN elastase by using an antihuman leukocyte elastase antibody. Secondly, the levels of PMN elastase-al proteinase inhibitor complex (EIC) in the blood and synovial fluid of 170 patients with Ri were measured by immunoassay. The results were as follows: 1, Gelatinolitic activity was shown to be mainly due to PMN elastase, and found to be highest in cartilage and synovia in RA joints, 2, The EIC levels in plasma of RA patients were significantly higher than those in gout and osteoarthritis (OA), and the EIC levels increased according to the stage of articular cartilage destruction. Moreover, the EIC levels in synovial fluid of RA patients were higher compared to those of OA patients, The activity of PMN elastase was elevated in destructive joints of Ri. With the progression of articular cartilage destruction, EIC levels in plasma of Ri patients increased as well, We suggest that PMN elastase may play a significant role in RA disease.
引用
收藏
页码:133 / 139
页数:7
相关论文
共 35 条
[1]  
ADEYEMI EO, 1990, BRIT J RHEUMATOL, V29, P15
[2]   POSSIBLE ROLE OF NEUTROPHIL PROTEINASES IN DAMAGE TO ARTICULAR-CARTILAGE [J].
BARRETT, AJ .
AGENTS AND ACTIONS, 1978, 8 (1-2) :11-18
[3]   INDUCTION OF NEUTROPHIL ENZYME-RELEASE BY RHEUMATOID FACTORS - EVIDENCE FOR DIFFERENCES BASED ON MOLECULAR CHARACTERISTICS [J].
BLACKBURN, WD ;
KOOPMAN, WJ ;
SCHROHENLOHER, RE ;
HECK, LW .
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1986, 40 (02) :347-355
[4]   BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF AZUROPHIL AND SPECIFIC GRANULES OF HUMAN NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES [J].
BRETZ, U ;
BAGGIOLINI, M .
JOURNAL OF CELL BIOLOGY, 1974, 63 (01) :251-269
[5]  
BROWN KA, 1988, BRIT J RHEUMATOL, V27, P150
[6]   RECOMBINANT HUMAN INTERLEUKIN-1 STIMULATES HUMAN ARTICULAR-CARTILAGE TO UNDERGO RESORPTION AND HUMAN CHONDROCYTES TO PRODUCE BOTH TISSUE-TYPE AND UROKINASE-TYPE PLASMINOGEN-ACTIVATOR [J].
CAMPBELL, IK ;
PICCOLI, DS ;
BUTLER, DM ;
SINGLETON, DK ;
HAMILTON, JA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 967 (02) :183-194
[7]  
CAUSTON TE, 1984, ARTHRITIS RHEUM, V27, P285
[8]   THE MEASUREMENT OF COLLAGENASE, TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP), AND COLLAGENASE TIMP COMPLEX IN SYNOVIAL-FLUIDS FROM PATIENTS WITH OSTEOARTHRITIS AND RHEUMATOID-ARTHRITIS [J].
CLARK, IM ;
POWELL, LK ;
RAMSEY, S ;
HAZLEMAN, BL ;
CAWSTON, TE .
ARTHRITIS AND RHEUMATISM, 1993, 36 (03) :372-379
[9]  
CURRIE MS, 1990, BLOOD, V75, P1699
[10]   NEUTRAL PROTEINASES IN HUMAN INTERVERTEBRAL DISC - ROLE IN DEGENERATION AND PROBABLE ORIGIN [J].
FUJITA, K ;
NAKAGAWA, T ;
HIRABAYASHI, K ;
NAGAI, Y .
SPINE, 1993, 18 (13) :1766-1773