Epithelial-macrophage-dendritic cell interactions impact alarmins expression in asthma and COPD

被引：20

作者：

Paplinska-Goryca, Magdalena ^{[1
]}

Misiukiewicz-Stepien, Paulina ^{[2
]}

Nejman-Gryz, Patrycja ^{[1
]}

Proboszcz, Malgorzata ^{[1
]}

Mlacki, Michal ^{[3
]}

Gorska, Katarzyna ^{[1
]}

Krenke, Rafal ^{[1
]}

机构：

[1] Med Univ Warsaw, Dept Internal Med Pulm Dis & Allergy, Banacha 1a, PL-02097 Warsaw, Poland

[2] Med Univ Warsaw, Postgrad Sch Mol Med, Warsaw, Poland

[3] OncoArendi Therapeut SA, Warsaw, Poland

来源：

CLINICAL IMMUNOLOGY | 2020年 / 215卷

关键词：

Alarmins; TSLP; IL-33; IL-25; Asthma; COPD; Epithelium; THYMIC STROMAL LYMPHOPOIETIN; INFLAMMATION; IL-33; IL-25; CYTOKINES; EXPANSION; RESPONSES; RECEPTOR;

D O I：

10.1016/j.clim.2020.108421

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the respiratory system macrophages and dendritic cells collaborate as sentinels against foreign particulate antigens. The study used a triple-cell co-culture model, utilizing nasal epithelial cells, along with: monocyte derived macrophages (moM phi s), and monocyte derived DCs (moDCs). Cell cultures from 15 controls, 14 asthma and 11 COPD patients were stimulated with IL-13 and poly I:C for 24 h. Co-cultivation of epithelial cells with moM phi s and moDCs increased TSLP level only in asthma and the effect of IL-13 and poly I:C stimulation differed in all groups. Asthma epithelial cells expressed higher level of receptors TSLPR, ST2 and IL-17RA than controls and increased number of ST2 + ciliated and IL17RA + secretory cells. Cytokine expression in respiratory epithelium may be influenced by structural and immunological cell interaction. TSLP pathway may be associated with secretory, while IL-33 with ciliated cells. The impaired function of respiratory epithelium may impact cell-to-cell interactions in asthma.

引用

页数：17

共 48 条

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