G2 rotavirus infections in an infantile population of the south of Italy:: Variability of viral strains over time

被引:28
作者
Arista, S
Giammanco, GM
De Grazia, S
Colomba, C
Martella, V
Cascio, A
Iturriza-Gòmara, M
机构
[1] Univ Palermo, Dipartimento Igiene & Microbiol, I-90127 Palermo, Italy
[2] Univ Palermo, Ist Patol Infettiva & Virol, Palermo, Italy
[3] Univ Bari, Dipartimento Sanita & Benessere Anim, Bari, Italy
[4] Univ Messina, Clin Malattie Infett, Messina, Italy
[5] Hlth Protect Agcy, Ctr Infect, Enter Virus Unit, Virus Reference Dept, London, England
关键词
rotavirus G2; genetic variation; VP7; protein; VP4; phylogenetic analysis;
D O I
10.1002/jmv.20496
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rotavirus positive samples collected in Palermo, Italy, during 2002-2004 did not react with the G2 type-specific RV5:3 monoclonal antibodies (MAbs) and could be identified as G2 only by RT-PCR genotyping. The genetic variation of VP7 and VP4 antigenic proteins was studied in 14 G2 samples including a selection of both those successfully characterized by serotyping and those failing to be serotyped. The phylogenetic analysis performed on partial VP7 sequences showed a temporal clustering of these strains, with those isolated in Palermo in 2003 belonging to the same lineage of G2 MAbs-unreactive strains identified in UK in 1996-1997 and in Bari, Italy, in 2003-2004. A single amino acid substitution in VP7 antigenic region A, at position 96 (Asp -> Asn), was consistently associated with the loss of antigenic reactivity. Five of the G2 strains were further characterized by sequencing of VP4-encoding genes as belonging to the P[4] type, and separate lineages clustering the strains according to a temporal distribution could be described. VP7 and VP4 antigenic proteins analysis provided evidence that over the last 11 years, at least two different populations of G2P[4] rotavirus strains have been infecting the infant population in Palermo. Considering the role of anti-VP7 and anti-VP4 neutralizing antibodies in rotavirus immunity, the emergence of new VP7-VP4 gene combinations might influence rotavirus circulation in the infant population and should be taken into consideration when devising vaccination strategies.
引用
收藏
页码:587 / 594
页数:8
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