Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii

被引：36
作者：
Arockiaraj, Jesu [1 ]
Easwvaran, Sarasvathi [1 ]
Vanaraja, Puganeshwaran [1 ]
Singh, Arun [2 ]
Othman, Rofina Yasmin [1 ]
Bhassu, Subha [1 ]
机构：
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Ctr Biotechnol Agr Res,Div Genet & Mol Biol, Kuala Lumpur 50603, Malaysia
[2] St Xaviers Coll Autonomous, Ctr Aquaculture Res & Extens, Palayankottai 627002, Tamil Nadu, India
来源：
FISH & SHELLFISH IMMUNOLOGY | 2012年 / 32卷 / 01期
关键词：
Caspase; Macrobrachium rosenbergii; IHHNV; Gene expression; Hydrolyzing activity; SHRIMP LITOPENAEUS-VANNAMEI; SPOT-SYNDROME-VIRUS; MOLECULAR-CLONING; WHITE SHRIMP; VIBRIO-ALGINOLYTICUS; APOPTOTIC PATHWAYS; CELL-DEATH; GENE; PROTEASE; ACTIVATION;
D O I：
10.1016/j.fsi.2011.11.006
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Caspase 3c (MrCasp3c) was sequenced from the freshwater giant prawn Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique. MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa. MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236-367 and 378-468 respectively. The quantitative real time PCR analysis revealed a broad expression of MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition, MrCasp3c was expressed in Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of M. rosenbergii. (C) 2011 Elsevier Ltd. All rights reserved.
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收藏
页码：161 / 169
页数：9
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