Interactions of protein kinase CK2β subunit within the holoenzyme and with other proteins

被引：47
|
作者：
Kusk, M
Ahmed, R
Thomsen, B
Bendixen, C
Issinger, OG
Boldyreff, B
机构：
[1] Odense Univ, Biokem Inst, DK-5230 Odense M, Denmark
[2] Danish Inst Anim Sci, Res Ctr Foulum, Tjele, Denmark
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 1999年 / 191卷 / 1-2期
关键词：
protein kinase CK2; subunit interaction; two-hybrid screening; p90(rsk); Doc-1; FAF1; IF-2mt; propionyl CoA carboxylase;
D O I：
10.1023/A:1006840613986
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Protein kinase CK2 is a ubiquitous, highly conserved protein kinase with a tetrameric alpha(2)beta(2) structure. For the formation of this tetrameric complex a beta-beta dimer seems to be a prerequisite. Using the two-hybrid system and a series of CK2 beta deletion mutants, we mapped domains involved in alpha-beta and beta-beta interactions. We also detected an intramolecular beta interaction within the amino acid stretch 132-165. Using CK2 beta as a bait in a two-hybrid library screening several new putative cellular partners have been identified, among them the S6 kinase p90(rsk), the putative tumor suppressor protein Doc-1, the Pas-associated protein FAF1, the mitochondrial translational initiation factor 2 and propionyl CoA carboxylase beta subunit.
引用
收藏
页码：51 / 58
页数：8
相关论文
共 50 条
  • [21] Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET2
    Hubner, Gerda M.
    Larsen, Jane Nohr
    Guerra, Barbara
    Niefind, Karsten
    Vrecl, Milka
    Issinger, Olaf-Georg
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2014, 397 (1-2) : 285 - 293
  • [22] Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET2
    Gerda M. Hübner
    Jane Nøhr Larsen
    Barbara Guerra
    Karsten Niefind
    Milka Vrecl
    Olaf-Georg Issinger
    Molecular and Cellular Biochemistry, 2014, 397 : 285 - 293
  • [23] Discrimination between the activity of protein kinase CK2 holoenzyme and its catalytic subunits
    Salvi, Mauro
    Sarno, Stefania
    Marin, Oriano
    Meggio, Flavio
    Itarte, Emilio
    Pinna, Lorenzo A.
    FEBS LETTERS, 2006, 580 (16): : 3948 - 3952
  • [24] Protein kinase CK2
    Pinna, LA
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1997, 29 (04): : 551 - 554
  • [25] Primary and secondary interactions between CK2α and CK2β lead to ring-like structures in the crystals of the CK2 holoenzyme
    Karsten Niefind
    Olaf-Georg Issinger
    Molecular and Cellular Biochemistry, 2005, 274 : 3 - 14
  • [26] Primary and secondary interactions between CK2α and CK2β lead to ring-like structures in the crystals of the CK2 holoenzyme
    Niefind, K
    Issinger, OG
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2005, 274 (1-2) : 3 - 14
  • [27] Protein Kinase CK2 in Health and DiseaseStructural bases of protein kinase CK2 inhibition
    R. Battistutta
    Cellular and Molecular Life Sciences, 2009, 66 : 1868 - 1889
  • [28] Environmental reprogramming of the expression of protein kinase CK2β subunit in fish
    Alvarez, M
    Kausel, G
    Figueroa, J
    Vera, MI
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2001, 227 (1-2) : 107 - 112
  • [29] Identification of new inhibitors of protein kinase CK2 subunit interaction
    Raaf, J.
    Neundorf, I
    Herzig, S.
    Issinger, O-G
    Niefind, K.
    Pietsch, M.
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2012, 385 : 69 - 69
  • [30] Environmental reprogramming of the expression of protein kinase CK2β subunit in fish
    Marco Alvarez
    Gudrun Kausel
    Jaime Figueroa
    María Inés Vera
    Molecular and Cellular Biochemistry, 2001, 227 : 107 - 112
12345