Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor

被引：203

作者：

Cui, Rongjing ^{[1
]}

Huang, Haiping ^{[1
]}

Yin, Zhengzhi ^{[1
]}

Gao, Di ^{[1
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 11期

基金：

中国国家自然科学基金;

关键词：

electrochemical biosensor; amplified immunoassay; hybrids;

D O I：

10.1016/j.bios.2008.01.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper describes the combination of electrochemical immunosensor using gold nanoparticles (GNPs)/carbon nanotubes (CNTs) hybrids platform with horseradish peroxidase (HRP)-functionalized gold nanoparticle label for the sensitive detection of human IgG (HIgG) as a model protein. The GNPs/CNTs nanohybrids covered on the glass carbon electrode (GCE) constructed an effective antibody immobilization matrix and made the immobilized biomolecules hold high stability and bioactivity. Enhanced sensitivity was obtained by using bioconjugates featuring HRP labels and secondary antibodies (Ab(2)) linked to GNPs at high HRP/Ab(2) molar ratio. The approach provided a linear response range between 0.125 and 80 ng/mL with a detection limit of 40 pg/mL. The immunosensor showed good precision, acceptable stability and reproducibility and could be used for the detection of HIgG in real samples, which provided a potential alternative tool for the detection of protein in clinical laboratory. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1666 / 1673

页数：8

共 25 条

[1] Double-codified gold nanolabels for enhanced immunoanalysis [J].