METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair

被引：8
作者：
Dai, Yarong [1 ]
Cheng, Maosheng [2 ,3 ]
Zhang, Siyan [1 ]
Ling, Rongsong [1 ]
Wen, Jieqi [1 ]
Cheng, Yifan [1 ]
Huang, Boxuan [1 ]
Li, Jinrong [4 ]
Dai, Caifeng [5 ]
Mao, Shiqing [1 ]
Lin, Shuibin [3 ]
Shen, Huangxuan [4 ]
Jiang, Yizhou [1 ]
机构：
[1] Shenzhen Univ, Inst Adv Study, Shenzhen 518067, Peoples R China
[2] Anhui Med Univ, Hefei 230032, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 1, Precis Med Inst, Ctr Translat Med, Guangzhou 510064, Peoples R China
[4] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510064, Peoples R China
[5] Shandong Univ, Qilu Hosp, Dept Obstet & Gynecol, Ctr Reprod Med, Jinan 250012, Peoples R China
来源：
STEM CELLS INTERNATIONAL | 2021年 / 2021卷
关键词：
GENE-THERAPY; METHYLATION; LIMBAL; AHNAK; MASS; DNA;
D O I：
10.1155/2021/5512153
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Limbal stem cells are essential for continuous corneal regeneration and injury repair. METTL3-catalyzed N6-methyladenosine (m(6)A) mRNA modifications are involved in many biological processes and play a specific role in stem cell regeneration, while the role of m(6)A modifications in corneal injury repair remains unknown. In this study, we generated a limbal stem cell-specific METTL3 knockout mouse model and studied the role of m(6)A in repairing corneal injury caused by alkali burn. The results showed that METTL3 knockout in the limbal stem cells promotes the in vivo cell proliferation and migration, leading to the fast repair of corneal injury. In addition, m(6)A modification profiling identified stem cell regulatory factors AHNAK and DDIT4 as m(6)A targets. Our study reveals the essential functions of m(6)A RNA modification in regulating injury repair and provides novel insights for clinical therapy of corneal diseases.</p>
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页数：14
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