A versatile clearing agent for multi-modal brain imaging

被引：196

作者：

Costantini, Irene ^{[1
]}

Ghobril, Jean-Pierre ^{[4
]}

Di Giovanna, Antonino Paolo ^{[1
]}

Mascaro, Anna Letizia Allegra ^{[1
]}

Silvestri, Ludovico ^{[1
]}

Muellenbroich, Marie Caroline ^{[1
]}

Onofri, Leonardo ^{[1
]}

Conti, Valerio ^{[6
,7
]}

Vanzi, Francesco ^{[1
,8
]}

Sacconi, Leonardo ^{[1
,2
]}

Guerrini, Renzo ^{[6
,7
]}

Markram, Henry ^{[4
]}

Iannello, Giulio ^{[5
]}

Pavone, Francesco Saverio ^{[1
,2
,3
]}

机构：

[1] Univ Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy

[2] CNR, Natl Opt Inst, I-50125 Florence, Italy

[3] Univ Florence, Dept Phys & Astron, I-50019 Sesto Fiorentino, Italy

[4] Ecole Polytech Fed Lausanne, Brain Mind Inst, Lab Neural Microcircuitry, CH-1015 Lausanne, Switzerland

[5] Univ Campus Biomed Rome, Dept Engn, I-00128 Rome, Italy

[6] Univ Florence, A Meyer Childrens Hosp, Dept Neurosci Pharmacol & Child Hlth, Pediat Neurol & Neurogenet Unit, I-50139 Florence, Italy

[7] Univ Florence, A Meyer Childrens Hosp, Dept Neurosci Pharmacol & Child Hlth, Labs, I-50139 Florence, Italy

[8] Univ Florence, Dept Biol, I-50125 Florence, Italy

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

MOUSE; 2-PHOTON; LIGHT; RECONSTRUCTION; TOMOGRAPHY; NEURONS; CLARITY; SCALE; MICE; VIVO;

D O I：

10.1038/srep09808

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Extensive mapping of neuronal connections in the central nervous system requires high-throughput mm-scale imaging of large volumes. In recent years, different approaches have been developed to overcome the limitations due to tissue light scattering. These methods are generally developed to improve the performance of a specific imaging modality, thus limiting comprehensive neuroanatomical exploration by multi-modal optical techniques. Here, we introduce a versatile brain clearing agent (2,2'-thiodiethanol; TDE) suitable for various applications and imaging techniques. TDE is cost-efficient, water-soluble and low-viscous and, more importantly, it preserves fluorescence, is compatible with immunostaining and does not cause deformations at sub-cellular level. We demonstrate the effectiveness of this method in different applications: in fixed samples by imaging a whole mouse hippocampus with serial two-photon tomography; in combination with CLARITY by reconstructing an entire mouse brain with light sheet microscopy and in translational research by imaging immunostained human dysplastic brain tissue.

引用

页数：9

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