Bioactive constituent production in St. John's Wort in vitro hairy roots. regenerated plant lines

被引：34

作者：

Bertoli, Alessandra ^{[1
]}

Giovannini, Annalisa ^{[2
]}

Ruffoni, Barbara ^{[2
]}

Di Guardo, Angela ^{[2
]}

Spinelli, Guido ^{[3
]}

Mazzetti, Michele ^{[3
]}

Pistelli, Luisa ^{[1
]}

机构：

[1] Univ Pisa, DCBB, I-56126 Pisa, Italy

[2] Ist Sperimentale Floricoltura San Remo, I-18038 Imperia, Italy

[3] Agenzia Reg Protez Ambientale ARPAT, Settore Chim Alimenti, I-57126 Livorno, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 13期

关键词：

H; perforatum; A; rhizogenes; ASE; HPLC-PDA-ESI-MS; chlorogenic acid; hyperforin; hypericin; hyperoside; quercetin; quercetrin; rutin;

D O I：

10.1021/jf0729107

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A wild strain of Agrobacterium rhizogenes was used to regenerate twelve in vitro plant lines from different hairy roots of H. perforatum (St. John's Wort). The production of the main bioactive constituents was observed even though their yields varied in the different plant lines. Two lines were selected for the hyperoside production (4.9-4.6 mg/g(dw)) while nine were characterized by significant yields of chlorogenic acid (ranged from 0.47 to 1.09 mg/gdw). Furthermore, one out of twelve lines showed a 10-fold higher hypericin content (0.25 mg/g(dw)) than that reported for the in vitro shoots in the literature. Morphological and phytochemical features were determined in order to select H. perforatum genotypes enriched in valuable bioactive compounds.

引用

页码：5078 / 5082

页数：5

共 31 条

[1] Determination of major constituents in St. John's Wort under different extraction conditions [J].