Performance of the Novel Qiagen artus QS-RGQ Viral Load Assays Compared to That of the Abbott RealTime System with Genetically Diversified HIV and Hepatitis C Virus Plasma Specimens

被引：10
作者：
Drexler, Jan Felix [1 ]
Reber, Ulrike [1 ]
Wuttkopf, Andrea [1 ]
Eis-Huebinger, Anna Maria [1 ]
Drosten, Christian [1 ]
机构：
[1] Univ Bonn, Med Ctr, Inst Virol, Bonn, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 06期
关键词：
TAQMAN HCV TEST; INTERNATIONAL UNITS; RNA; SERUM; QUANTIFICATION; QUANTITATION; EXTRACTION; INFECTION; THERAPY; TYPE-1;
D O I：
10.1128/JCM.05874-11
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We compared two novel Qiagen QS-RGQ viral load assays with the established Abbott RealTime assays on a highly diversified panel of 121 human immunodeficiency virus (HIV) and 107 hepatitis C virus (HCV) specimens. The quantifications correlated well for all HIV and HCV types, but Qiagen yielded higher HCV concentrations than Abbott, predominantly in genotypes 4 and 5.
引用
收藏
页码：2114 / 2117
页数：4
相关论文
共 18 条
  • [1] [Anonymous], 2004, VERBOT VERWENDUNG HP, V166, P19770
  • [2] Performance of the new Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: Conversion to international units and comparison with the Roche COBAS amplicor HCV monitor, version 2.0, assay
    Beld, M
    Sentjens, R
    Rebers, S
    Weegink, C
    Weel, J
    Sol, C
    Boom, R
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) : 788 - 793
  • [3] Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method
    Chevaliez, Stephane
    Bouvier-Alias, Magali
    Brillet, Rozenn
    Pawlotsky, Jean-Michel
    [J]. HEPATOLOGY, 2007, 46 (01) : 22 - 31
  • [4] Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil
    Drexler, Jan Felix
    de Souza Luna, Luciano Kleber
    Pedroso, Celia
    Pedral-Sampaio, Diana Brasil
    Queiroz, Artur T. L.
    Brites, Carlos
    Netto, Eduardo M.
    Drosten, Christian
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) : 2061 - 2063
  • [5] A Novel Diagnostic Target in the Hepatitis C Virus Genome
    Drexler, Jan Felix
    Kupfer, Bernd
    Petersen, Nadine
    Tommasini Grotto, Rejane Maria
    Corvino Rodrigues, Silvia Maria
    Grywna, Klaus
    Panning, Marcus
    Annan, Augustina
    Silva, Giovanni Faria
    Douglas, Jill
    Koay, Evelyn S. C.
    Smuts, Heidi
    Netto, Eduardo M.
    Simmonds, Peter
    de Moura Campos Pardini, Maria Ines
    Roth, W. Kurt
    Drosten, Christian
    [J]. PLOS MEDICINE, 2009, 6 (02) : 210 - 220
  • [6] HIV-1 viral load assays for resource-limited settings
    Fiscus, Susan A.
    Cheng, Ben
    Crowe, Suzanne M.
    Demeter, Lisa
    Jennings, Cheryl
    Miller, Veronica
    Respess, Richard
    Stevens, Wendy
    [J]. PLOS MEDICINE, 2006, 3 (10) : 1743 - 1750
  • [7] Gelderblom HC, 2006, ANTIVIR THER, V11, P95
  • [8] Evaluation of the Roche Cobas TaqMan and Abbott RealTime extraction-quantification systems for HIV-1 subtypes
    Gueudin, Marie
    Plantier, Jean Christophe
    Lemee, Veronique
    Schmitt, Marie Paule
    Chartier, Loic
    Bourlet, Thomas
    Ruffault, Annick
    Damond, Florence
    Vray, Muriel
    Simon, Francois
    [J]. JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2007, 44 (05) : 500 - 505
  • [9] Improved version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for hepatitis C virus RNA: Calibration to international units, enhanced genotype reactivity, and performance characteristics
    Lee, SC
    Antony, A
    Lee, N
    Leibow, J
    Yang, JQ
    Soviero, S
    Gutekunst, K
    Rosenstraus, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) : 4171 - 4179
  • [10] Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5′ untranslated region sequences
    Murphy, Donald G.
    Willems, Bernard
    Deschenes, Marc
    Hilzenrat, Nir
    Mousseau, Roger
    Sabbah, Sidney
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (04) : 1102 - 1112
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