Evolution of the tRNATYr/TyrRS aminoacylation systems

被引：50

作者：

Bonnefond, L ^{[1
]}

Giegé, R ^{[1
]}

Rudinger-Thirion, J ^{[1
]}

机构：

[1] CNRS, Inst Biol Mol & Cellulaire, UPR 9002, Dept Mecanismes & Macromol Synth Prot & Cristallo, F-67084 Strasbourg, France

来源：

BIOCHIMIE | 2005年 / 87卷 / 9-10期

关键词：

mitochondria; phylogeny; species-specificity; tyrosine identity;

D O I：

10.1016/j.biochi.2005.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tRNA identity rules ensuring fidelity of translation are globally conserved throughout evolution except for tyrosyl-tRNA synthetases (TyrRSs) that display species-specific tRNA recognition. This discrimination originates from the presence of a conserved identity pair, G1-C72, located at the top of the acceptor stem of tRNA(Tyr) from eubacteria that is invariably replaced by an unusual C1-G72 pair in archaeal and eubacterial tRNA(Tyr). In addition to the key role of pair 1-72 in tyrosylation, discriminator base A73, the anticodon triplet and the large variable region (present in eubacterial tRNA(Tyr) but not found in eukaryal tRNA(Tyr)) contribute to tyrosylation with variable strengths. Crystallographic structures of two tRNA(Tyr)/TyrRS complexes revealed different interaction modes in accordance with the phylum-specificity. Recent functional studies on the human mitochondrial tRNA(Tyr)/TyrRS system indicates strong deviations from the canonical tyrosylation rules. These differences are discussed in the light of the present knowledge on TyrRSs. (C) 2005 Elsevier SAS. All rights reserved.

引用

页码：873 / 883

页数：11

共 93 条

[1] A PROTEIN REQUIRED FOR SPLICING GROUP-I INTRONS IN NEUROSPORA MITOCHONDRIA IS MITOCHONDRIAL TYROSYL-TRANSFER RNA-SYNTHETASE OR A DERIVATIVE THEREOF [J].