Characterisation of CMY-4, an AmpC-type plasmid-mediated p-lactamase in a Tunisian clinical isolate of Proteus mirabilis

被引：34
作者：
Verdet, C
Arlet, G
Ben Redjeb, S
Ben Hassen, A
Lagrange, PH
Philippon, A
机构：
[1] Univ Paris 07, Hop St Louis, Microbiol Serv, F-75475 Paris, France
[2] Hop Charles Nicolle, Microbiol Serv, Tunis, Tunisia
来源：
FEMS MICROBIOLOGY LETTERS | 1998年 / 169卷 / 02期
关键词：
beta-lactamase; cefoxitin resistance; Proteus mirabilis;
D O I：
10.1111/j.1574-6968.1998.tb13323.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A strain of Proteus mirabilis resistant to beta-lactams, including cefoxitin, was isolated from the urine of a woman from Tunisia. Its antibiotic susceptibility pattern and that of the Escherichia coli transconjugant suggested the presence of an AmpC-type beta-lactamase. Two bands of beta-lactamase activity (pI 5.4 and 9.2) were detected by isoelectric focusing. The nucleotide sequence of the gene encoding the AmpC-type enzyme was determined. The deduced amino acid sequence was 98-99% identical to CMY-3 and to those of the plasmid-mediated AmpC-type beta-lactamases originated from Citrobacter freundii and 97% identical to the chromosome-encoded beta-lactamase of a Tunisian clinical isolate of C. freundii. This enzyme differs from CMY-2 by one substitution (Arg for Trp at position 221) and From CMY-3 by two substitutions (Glu for Gly at position 42 and Ser for Asn at position 363) and we propose the denomination CMY-4. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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页码：235 / 240
页数：6
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