Diversity of the human intestinal microbial flora

被引：5894
作者：
Eckburg, PB
Bik, EM
Bernstein, CN
Purdom, E
Dethlefsen, L
Sargent, M
Gill, SR
Nelson, KE
Relman, DA
机构：
[1] Stanford Univ, Sch Med, Div Infect Dis & Geog Med, Stanford, CA 94305 USA
[2] Dept Microbiol & Immunol, Stanford, CA 94305 USA
[3] Univ Manitoba, Dept Med, Gastroenterol Sect, Winnipeg, MB R3A 1R9, Canada
[4] Stanford Univ, Dept Stat, Stanford, CA 94305 USA
[5] Inst Genom Res, Rockville, MD 20850 USA
[6] Vet Affairs Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA
来源：
SCIENCE | 2005年 / 308卷 / 5728期
关键词：
D O I：
10.1126/science.1110591
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The human endogenous intestinal microflora is an essential "organ" in providing nourishment, regulating epithelial. development, and instructing innate immunity; yet, surprisingly, basic features remain poorly described. We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity. A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms. We discovered significant intersubject variability and differences between stool and mucosa community composition. Characterization of this immensely diverse ecosystem is the first step in elucidating its role in health and disease.
引用
收藏
页码：1635 / 1638
页数：4
相关论文
共 22 条
[1]   The gut microbiota as an environmental factor that regulates fat storage [J].
Bäckhed, F ;
Ding, H ;
Wang, T ;
Hooper, LV ;
Koh, GY ;
Nagy, A ;
Semenkovich, CF ;
Gordon, JI .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (44) :15718-15723
[2]   Phylogenetic relationships of butyrate-producing bacteria from the human gut [J].
Barcenilla, A ;
Pryde, SE ;
Martin, JC ;
Duncan, SH ;
Stewart, CS ;
Henderson, C ;
Flint, HJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) :1654-1661
[3]  
Colwell R. K., 2004, ESTIMATES VERSION 7
[4]   Prokaryotic diversity and its limits: microbial community structure in nature and implications for microbial ecology [J].
Curtis, TP ;
Sloan, WT .
CURRENT OPINION IN MICROBIOLOGY, 2004, 7 (03) :221-226
[5]   Community assembly and invasion: An experimental test of neutral versus niche processes [J].
Fargione, J ;
Brown, CS ;
Tilman, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (15) :8916-8920
[6]   Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods [J].
Hayashi, H ;
Sakamoto, M ;
Benno, Y .
MICROBIOLOGY AND IMMUNOLOGY, 2002, 46 (08) :535-548
[7]  
Hold GL, 2002, FEMS MICROBIOL ECOL, V39, P33, DOI 10.1111/j.1574-6941.2002.tb00904.x
[8]   Commensal host-bacterial relationships in the gut [J].
Hooper, LV ;
Gordon, JI .
SCIENCE, 2001, 292 (5519) :1115-1118
[9]   Molecular analysis of commensal host-microbial relations hips in the intestine [J].
Hooper, LV ;
Wong, MH ;
Thelin, A ;
Hansson, L ;
Falk, PC ;
Gordon, JI .
SCIENCE, 2001, 291 (5505) :881-884
[10]   An ecological perspective on bacterial biodiversity [J].
Horner-Devine, MC ;
Carney, KM ;
Bohannan, BJM .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2004, 271 (1535) :113-122
123