Injectable supramolecular hydrogels based on host-guest interactions with cell encapsulation capabilities

被引:18
作者
Ren, Pengfei [1 ]
Wei, Dandan [1 ]
Ge, Xin [1 ]
Wang, Faming [1 ]
Liang, Min [1 ]
Dai, Jidong [1 ]
Xu, Li [1 ]
Zhang, Tianzhu [1 ]
机构
[1] Southeast Univ, Sch Biol Sci & Med Engn, Natl Demonstrat Ctr Expt Biomed Engn Educ, State Key Lab Bioelect, Nanjing 210096, Peoples R China
关键词
Supramolecular hydrogels; Host-guest interactions; Injectability; Shear-thinning; Tissue adhesion; Cell encapsulation; SHEAR-THINNING HYDROGELS; SELF-HEALING HYDROGELS; ALGINATE HYDROGELS; DRUG-DELIVERY; MATRIX; GEL; IMMUNOGENICITY; ADHESIVE;
D O I
10.1016/j.colsurfa.2021.127338
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Injectable hydrogels, which can deliver cells or drugs directly to tissue sites by minimally invasive injection methods, have attracted considerable attention. In this work, using beta-cyclodextrin-grafted alginate as the host polymer and adamantane-grafted polyacrylamide as the guest polymer, an injectable supramolecular hydrogel system was developed based on the host-guest interaction between beta-cyclodextrin (beta CD) and adamantane (Ad). Simple physical mixing and stirring methods allowed the hydrogels to be prepared in a short time (<1 min). The rheological and mechanical properties and microscopic morphology of the hydrogels were conveniently regulated by changing the grafting degree of beta CD and Ad in alginate or polyacrylamide. The shear-thinning and self healing properties induced by reversible host-guest interactions and a natural, plant-based polysaccharide endow the developed hydrogels with good injectability and biocompatibility, respectively. Experiments showed that the hydrogels effectively adhered to pork tissue. Moreover, human umbilical vein endothelial cells and human ovarian cancer cells encapsulated in the hydrogels showed good viability and three-dimensional distribution after being cultured for three days. Overall, the rapid preparation method and the advantages of injectability, self-healing and cytocompatibility endow the proposed injectable hydrogel system with great potential as a tissue engineering material.
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页数:13
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